To use this principle, but a day earlier, from the stage of isolation on a Petri dish, Dr. Alain Rambach then invented, and patented in 1979, a culture medium to differentiateEscherichia colibacteria by staining colonies, by incorporating a specific chromogenic substrate into the agar medium.
Later, in 1989, he successfully commercialized the first chromogenic culture medium accepted by the market, Rambach™ Agar forSalmonella.
In 1993, he founded the company CHROMagar™ to continue developing new chromogenic culture media to detect various microorganisms and innovate in the field of bacterial diagnostics.
In 1994, he developed the first CHROMagar™ bichromogenic medium: by combining two chromogens, he obtained a range of colony colorations, and the medium can differentiate a whole series of microorganisms on a single Petri dish.
On the same principle, the CHROMagar™ Candida medium is created to identify different species of yeast.