To use this principle,but a day earlier,from the stage of isolation on a Petri dish,Dr.Alain Rambach then invented,and patented in 1979,a culture medium to differentiateEscherichia colibacteria by staining colonies,by incorporating a specific chromogenic substrate into the agar medium。
Later,in1989,he successfully commercialized the first chromogenic culture medium accepted by the market,Rambach™,™Agar forSalmonella。
In1993,he founded the company CHROMagar™,™to continue developing new chromogenic culture media to detect various microorganisms and innovate in the field of bacterial diagnostics。
In1994,he developed the first CHROMagar™,™ bichromogenic medium:by combining two chromogens,he obtained a range of colony colorations,and the medium can differentiate a whole series of microorganisms on a single Petri dish。
On the same principle,the CHROMagar™,™ Candida medium is created to identify different species of yeast。