热固化(法)E.coli)was invented and patented by Dr Alain Rambach in1979。The introduction of this medium triggered arevolution in microbial diagnosis driven by the introduction of awhole range of media for the detection of key clinical&food borne pathogens。
How does Chromogenic Culture Media technology work?
The power of chromogenic technology is the combination of the detection of precise enzyme activity and localized staining precipitating in the colony,allowing an easy differentation of microorganisms thanks to the coloration of grown bacteria。
This technology is based on soluble colorless molecule called chromogen,composed of a substrate,targeting a specific enzymatic activity and a chromophore。
When the target organism’s enzyme cleaves the colorless chromogenic conjugate,the chromophore is released。Inits unconjugated form,the chromophore exhibits its distinctive colorand,due to reduced solubility,forms a precipitate。
The result is a very specific&distinctive color-based differentiation,which is clearly distinguishable to the naked eye under normal lighting conditions。