The QuickExtract™RNA Extraction Kit is a fast，simple way of preparing RNA for RT-PCR（both end-point and real-time）。The single-tube system requires only vortex mixing to lyse the cells，and prepare the RNA for cDNA synthesis.The result is easy processing of one to hundreds of samples in minutes，with no sample loss or toxic organic solvents。The QuickExtract RNA Extraction Solution works with cultured and suplesion，incells， has been tested on human，mouse，rat，E.coli，andS.aureuscell cultures.It is not suitable for tissue samples or plant samples.An optional DNase I treatment may improve certain downstream applications。
应用程序，应用程序
- Preparation of RNA from cultured adherent and suspension cells for RT-PCR。
 | Figure1.End-point RT-PCR of different regions of a14kb message using HeLa cell extract with the QuickExtract RNA Extraction Kit。A sample containing 105HeLa cells was lysed in 100µL of QuickExtract RNA Extraction Solution by vortex mixing。The lysate was reverse transcribed with the MMLV Reverse Transcriptase 1st Strand cDNA Synthesis Kit using standard conditions and random primers.The cDNA was then amplified with six primer sets to p532using the FailSafe™PCR System。Lane M，100bp ladder；Lane112984to13892；lane29406to10202；lane35194to5802；lane44191to4690；lane52280to2676；and lane61029to1329。 |
 | Figure2.Comparative yield of RT-PCR product with different RNA extraction kits。Lysates were prepared according to manufacturers'instructions and used as template to produce cDNA using the MMLV RT 1st Strand cDNA Synthesis Kit，followed by PCR using the FailSafe PCR System with primers for the ALDOA gene.Products were separated on a2%agarose gel and stained with SYBR®gold.Lane M，100bp ladder；lanes1and2，QuickExtract RNA Extraction Kit；lanes3and4，kit from Vendor1；lanes5and6，kit from Vendor2。 |
