OligoSpec计算器

The OligoSpec™，™calculator provides the physical properties for your oligo design。Quickly calculate your oligo’smolecular weight and extinction coefficient。

5’修改

顺序，顺序内部导航

3’修改

calculate

Illegal sequence！

BTI extinction Co.260nm：{ecmwCtrl.btiCo}}

Backbone MW calc:{ecmwCtrl.backbone}}

Protonated MW calc：{ecmwCtrl.protonated}}

Counter ion MW calc:{ecmwCtrl.ion}

入口notes

The OligoSpec calculator outputs the physical properties for a particular oligo design。外插式计算-外插式计算：ε260=[（Sum ofε260 for all bases*）+（ε260for all modifications*）]x0.9，to adjust for hyperchromicity。

Where:

ε260 of bases=dA（ε260=15200），dC（ε260=7050），dG（ε260=120010），and T（ε260=8400）。
全部，全部ε260气门传动装置^-1•cm^-1

*所有车辆都有可能发生故障oligo modifications page本发明的技术规范downloading this PDF。

照明计算-
Molecular weight backbone（g/mol）is calculated using the following formula:MW（backbone）=∑（nbase*MWbase）+MW Modifications+（nPO2*MWPO2）+MWHadj

Where:

Wbase=molecular weight of the deprotonated nucleoside。
WMod=molecular weight of the deprotonated form of each individual modification
nPO2=total count of internal phosphates，i.e.，nbases+nmods-1。
WPO2=molecular weight of PO2，i.e.，62.97。This is is the MW for that portion of a phosphate linkage not already included in MWbase or MWmod。
WHadj=0,1，or2protons（i.e.，0,1.01,or2.02），depending on the presence and structure of 3'and5'modifications。

The addition of counter ions is simply the molecular weight backbone plus the total weight of counter ions。This added weight is typically equal to the number of phosphates in the oligo multiplied by the weight of the counter ion。The weight of each counter ion is generally assumed to 102.2 for Triethylammonium（TEA）。Note that some modifications add additional phosphate groups to the molecular weight calculation，such asa3’phosphate modification or3’thymidine linked modifications。

The protonated molecular weight is also typically equal to the backbone weight plus the total number of phosphates multiplied by1.01for each proton（H+）。This is the molecular weight that is typically observed by electrospray mass spectrometry run in negative ion mode。