Capture-Seq

Capture-Seq is a targeted next generation sequencing（NGS）platform forcharacterising hundreds of thousands of targets（including complex polyploids）。Samples may be comprised of a single species，a mix of closely related species or distantly related taxa。Capture-Seq is suitable for both high and low volume applications。

Finding needles in the genomic haystack

Capture-Seq is the most comprehensive targeted genotyping system for discovering and characterising the genome for：

Allele mining:Pre-breeding marker discovery
SNP genotyping and haplotyping
QTL mapping and candidate gene identification
Genetic fingerprinting
多边形平面照明
RenSeq Resistance gene sequencing
Pan-genome construction and mapping
Any organism can be analysed using Capture-Seq，and we offer a suite of validated，ready-made Capture-Seq panels for industry-wide standardisation of genotyping data。

连接到轨道

资源，资源

目标信号序列

水力调节器
可预应力和可调节面板
Legacy data compatible
短距离、长距离、短距离
Illumina®
PacBio®

Tissue/DNA to Fastq data，SNPs and more

最小500 samples for DNA extraction
上方向5-week turnaround times

可重复的，可重复的

Sequence the same regions every time
<1%missing target data

可拆卸的、可拆卸的、可拆卸的

低minimum sample number
最小ascertainment bias

Capture-Seq vs arrays

Genotyping system	目标genotyping data	数据输入装置	开发成本	出口和出口	缺少数据%	Ascertainment bias	回切值选择（Readdepth at targets）	可修复性	不可安装的车辆显示器	haplotype reconstruction	Ease if copy number and dosage detection	分析complexity
Capture-Seq	是	400-500bp	低，低	高电平	低，低	低，低	可预制和可控制的	高电平	是	是	高电平	低，低
Chip-based arrays	是	1bp	高电平	低，低	低，低	高电平	N/A	高电平	否	否	低，低	低，低

Capture-seq technology

Answering multiple questions in tandem

Capture-Seq flexibly targets SNPs，genes，and QTLs at the same time to produce sequence data at each region，instead of a single SNP，through the power of NGS。This enables Capture-Seq to provide greater genomic insights into breeding material and training populations，including the ability to phase alleles into haplotypes formore effective polyploid results。

Easy and adaptive

Capture-Seq panels are suitable for different breeding programs of the same or closely related species，ensuring comprehensive pre-breeding marker discovery with minimal risk of ascertainment bias and wasted resources。

捕获信号灯

Capture-Sequses targeted probe hybridisation technology to selectively recover regions of interest throughout the genome of any species。

Capture-Seq probe targets are selected from reference sequence data including genomes and/or transcriptomes。After target selection，Biosearch Technologies designs and synthesises complementary probe sequences to enrich for target regions during sample processing。
Genomic DNA is processed to sequencing-compatible DNA libraries。
After DNA library preparation，Capture-Seq probes are hybridised to each sample to selectively enrich target regions over other non-targeted DNA sequences。This improves the proportion of sequencing data going towards regions of interest vs the overall genome。
After next generation sequencing（NGS），sample data from the target regions is analysed and markers are identified across all samples。
Markers can be further analysed foruse in genomic selection，genome-wide association studies，insertion site characterisations and more。

Click to view diagram

Capture-seq genotyping results

规格，规格	Genotyping objective	#Capture-Seq targets	#SNPs detected	缺少数据%
Maize	Fine mapping resistance genes（R-genes）	5000	4623	0.15
Sugarcane	Genomic selection and genetic mapping	10000	37976	0.1
下部，下部	Genomic selection	20000	67525	0.002
Blueberry	Genomic selection and genetic mapping	31000	205057	0.03