For SNP discovery and characterising hundreds of thousands of targets of any species（including complex polyploids）check out Capture-Seq。
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Flex-Seq technology
多层次结构>PCR
The secret is Flex-Seq probe technology
- 高强度混凝土浇注板
- Higher marker recovery than multiplexed PCR
- Greater flexibility to include targets than chip-based arrays
SNPs within a300bp window may be suitable for haplotyping。Phased alleles improve breeding selections，especially in polyploid species。
Flex-Seq targets individual regions using two Flex-Seq probes，often focusing on a single SNP。Probes can be designed to flexibly bind on either side of the SNP，leading to greater ability to recover desired targets。Once both probes bind，the complementary sequence is synthesised between the probes and the target region is amplified from the genomic DNA。The genomic DNA and non-specific binding products are removed。The proprietary Flex-Seq probes enable high levels of target multiplexing in a single reaction。
柔性密封性能
Summary metrics of genotyping results from three Flex-Seq marker panels。
数据Uniformity displays the percentage of markers with>0.2x mean sequence depth（91%-98%）。
Markers Recovered shows the percentage of markers（92%-98%）with sufficient sequencing depth for accurate genotyping（i.e.10X per haploid genome）。
Sequencing reads on Target reflects the reaction specificity，with95-99%of all aligned sequencing data mapping to the intended targets。
The combination of high marker recovery，data uniformity and reaction specificity ensures efficiency and overall completeness of genotyping results for any marker panel。
Flex-Seq results uniformity
Log transformation of sequencing depth per marker using three different marker panels。统一form sequencing depth across all markers enables efficient multiplexed sequencing of thousands of samples。
These efficiencies can be replicated in novel marker panel designs to enable customised targeting of thousands of markers across the genome。
柔性密封性
Correlation of the percentage of reads generated per marker between technical replicates using three different marker panels。Flex-Seq demonstrates high levels of consistency in marker recovery and data quantity per marker from independent sample reactions。High repeatability translates to a more predictable recovery of markers from sample to sample，improving the ease of additional data analysis and completeness of final results。
