Skip to content Skip to navigation menu
cancel
  1. 主页
  2. Molecular biology enzymes
  3. 空的，空的
  4. RNA Nucleases
  5. Hybridase Thermostable RNase H

Hybridase Thermostable RNase H

Hybridase Thermostable RNase H，5U/µL500U

Hybridase Thermostable RNase H

Hybridase Thermostable RNase H，5U/µL500U

Specifically degrade the RNA in a DNA：RNA hybrid，without affecting DNA or unhybridised RNA，at higher reaction temperatures。

关键帧features

显示，显示 弯头，弯头
  • 特定degrade the RNA in a DNA：RNA hybrid，without affecting DNA or unhybridised RNA，at higher reaction temperatures
  • Optimal activity above 65℃and maintains activity as high as 95℃
  • RNA in a RNA:DNA hybrid and will not digest free RNA or DNA
  • 主要决策和决策while minimising background due to nonspecific hybridisiation
选择大小
项目标识 H39500
TBD
添加到basket to request a quote

产品说明

Hybridase Thermostable RNase Hspecifically degrades the RNA in a DNA：RNA hybrid，without affecting DNA or unhybridized RNA.In contrast toE.coliRNase H，which is rapidly inactivated at55°C，Hybridase RNase H is active at high temperatures.It has optimal activity above65°C and can be used at temperatures up to 95°C。The thermostability of the enzyme permits it to be used at temperatures that give the highest hybridisation stringncy for specific DNA：RNA heteroduplexes，maximizing sensitivity and selectivity while minimizing background due to nonspecific hybridization。

应用程序，应用程序

  • High-stringency hybrid selection。
  • 诊断assays of specific target DNA sequences by isothermal probe amplification。
  • Transcription-based amplification methods（e.g.，NASBA®method）。
  • High-stringency mapping of mRNA structure。
  • Applications that require specific hydrolysis of the RNA in a DNA：RNA hybrid。

Unit Definition：One unit of Hybridase RNase H results in the acid-solubilization of 1nmol of polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20minutes at45°C under standard assay conditions。

Note： The unit assay is performed at 45°C because this is optimal for the Tmof poly（dT）：poly（A）。The optimal temperature for many applications may be considerably high er。

存储缓冲器：50%glycerol containing 50mM Tris-HCl（pH7.5），0.1M NaCl，1.0mM DTT，0.1mM EDTA，and 0.1%Triton®X-100。

质量控制：Hybridase Thermostable RNase H is tested for RNA degradation in a RNA:DNA hybrid and for the absence of detectable exo-or endodeoxyribonuclease，and non-RNase H RNase activities。

SDS

手动和用户指南

访问支持

Need some support with placing an order，setting up an account，or finding the right protocol？

Contact us