Multi-laboratory Validation Study of the Vitrigel-Eye Irritancy Test Method as an Alternative to In Vivo Eye Irritation Testing H.Kojima, H.Yamaguchi, T.Sozu, N.Kleinstreuer, L.Chae-Hyung, W.Chen, M.Watanabe, T.Fukuda, K.Yamashita, T.Takezawa Alternatives to Laboratory Animals, 47(3-4),140-157(2019).
Development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing eye irritant chemicals T.Takezawa, K.Nishikawa, Pi-Chao.Wang Toxicology in Vitro,25(6), 1237-1241(2011).
Prediction of Human Hepatic Clearance for Cytochrome P450 Substrates via a New Culture Method Using the Collagen Vitrigel Membrane Chamber and Fresh Hepatocytes Isolated from Liver Humanized Mice R.Watari, M.Kakiki, C.Yamasaki, Y.Ishida, C.Tateno, Y.Kuroda, S.Ishida, K.Kusano Biological and Pharmaceutical Bulletin, 42(3), 348-353(2019).
A long-term culture system based on a collagen vitrigel membrane chamber that supports liver-specific functions of hepatocytes isolated from mice with humanized livers R.Watari, M.Kakiki, A.Oshikata, T.Takezawa, C.Yamasaki, Y.Ishida, C.Tateno, Y.Kuroda, S.Ishida, K.Kusano The Journal of Toxicological Sciences,43(8), 521-529(2018).
Development of an oxygenation culture method for activating the liver-specific functions of HepG2 cells utilizing a collagen vitrigel membrane chamber A.Oshikata-Miyazaki, T.Takezawa Cytotechnology,68(5), 1801-1811(2016).
药剂的角膜透过性试验
Fabrication of a Corneal Model Composed of Corneal Epithelial and Endothelial Cells via a Collagen Vitrigel Membrane Functioned as an Acellular Stroma and Its Application to the Corneal Permeability Test of Chemicals H.Yamaguchi, T.Takezawa Drug Metabolism and Disposition,46(11), 1684-1691(2018).