Flow cytometry permits reliable analysis of cell phenotypes and is a workhorse technique in immunology,haematology,research and clinical diagnosis。
Cell-permeant DNA dyes can be used to discriminate nucleated from anucleated cells reducing the need for time-consuming and potentially damaging RBC lysis reagents,which can be important for precious or small samples。
Further,stoichiometric DNA labeling can further provide information on cell cycle status,ploidy and proliferation(S-phase fraction)without permeabilization or RNase treatment。
Clinical samples often need a viability dye to evaluate sample integrity on analysis。