产品回顾
What our customers are saying about APOPTRAK™,™
The Science
APOPTRAK™,™is a small molecule(442Da)belonging to the anthraquinone family。It is optimally excited at633or647nm and emission in the far-red/near infra-red(NIR)peaking at700nm。
APOPTRAK™,™流动cytometry应用程序:
- APOPTRAK™,™自行上xicity dye with similar spectral properties to DRAQ5™,™but with a reduced ability to bind to cellular DNA。Being charge neutral APOPTRAK™,™is capable of entering viable cells at low but detectable rates to primarily occupy cytoplasmic compartments。
- APOPTRAK™’sfar-red florescence signal in‘intact/live’cells increases when there is greater dye access upon the disruption of the plasma membrane。Residual DNA binding capacity of APOPTRAK™,™permits the detection of the nuclear fragments during cell break-up。
Why APOPTRAK™?
APOPTRAK™,™benefits from all the advantageous“far-red”spectral properties of its parent,but differs significantly in its rate of entry into LIVE cells。APOPTRAK™,™enters LIVE cells slowly and apoptotic cells rapidly,allowing a positive discrimination between these two populations。Key benefits include:-
- Different rate of entry into LIVE and apoptotic cells,allowing positive discrimination by flow cytometry(with Annexin V-FITC,for example)
- Easy to use-no lyse,no wash,no RNase needed
- Ideal for use with GFP&FITC labels-APPTRAK™,™fluoresces in the far-red region
APOPTRAK™,™can bring significant benefits to your application:
in FLOW CYTOMETRY:
- positive discrimination between intact cells,membrane-compromised cells,cellular debris
- 不动轴V binding assay protocols
技术信息
APOPTRAK™,™enters LIVE cells slowly and leaky(apoptotic)cells rapidly,to give a signal intensity difference of between5-and10-fold,depending on the cell type and treatment。This allows a positive discrimination between these two populations as shown in the example data below。
Published data(Wiltshire et al,Cytometry2000)suggests an ability of APOPTRAK™,™to discriminate early and late apoptotic cells when used in combination with Annexin V.The example below shows the uptake of APOPTRAK™,™in a B cell lymphoma sample carrying a low burden of apoptotic cells。Cells were co-stained with FITC-Annexin V。
- Exλmax633nm
- Emλmax700.5nm,with a red-shift of 12nm upon DNA binding
- Suggested optimal emission filters include Cy5695LP730/50BP or 780/60BP