The Science
- DRAQ5™,™is a small molecule(412DA)belonging to the anthraquinone family。It has peak absorbances at600nm and 646nm。It fluoresces in the far-red/near infra-red(NIR)peaking at697nm(bound to dsDNA)。电流互感器it possible to detect DRAQ5™,™using blue laser(488nm)excitation。
- DRAQ5™,™readily permeates the lipid bilayer of cell membranes and intercalates with high affinity to dsDNA。There is no fluorescence enhancement on binding,thus the measured fluorescence is proportional and stoichiometric to the quantity of nuclear DNA present in the cell。There is no measurable binding to mtDNA(van Zandvoort,2002)and DRAQ5™,™shows negligible binding to RNA。
- DRAQ5is highly photo-stable(Martin,2005)。
Why DRAQ5™?
There are many reasons why DRAQ5™,™is the DNA dye of choice:-
IT’S EASY-ready-to-use,straight from the fridge without the complications of DMSO,thawing,aliquoting
IT’S FLEXIBLE-a cross-platform DNA dye that works on live or fixed cell and tissue preparations
IT’S RELIABLE-demonstrated on hundreds of cell and tissue types and remarkably photostable
IT’S PROVEN预应力混凝土地基
DRAQ5™,™can bring significant benefits to your application:
in FLOW CYTOMETRY:
- 管径测量装置
- labeling of dsDNA is stoichiometric so you can measure DNA content/cell cycle position cell-by-cell
IMAGING FLOW CYTOMETRY:
- clear nuclear segmentation in the far-red for morphometrics and multi-colour analysis
in FLUORESCENCE MICROSCOPY:
- skip the queue for the UV-equipped microscope with this far-red counterstain!
in HIGH CONTENT SCREENING:
- 可观测的轨道流动性
- more info from the single channel nucl:cyto segmentation
in IN-CELL WESTERNS:
- 预应力混凝土施工法
技术信息
- DRAQ5™,™is supplied as adeep blue aqueous solution in two concentrations:0.5mM and 5mM。
- Products are shipped at ambient temperature,but on receipt packs should be stored at2-8°C。Do NOT freeze!DRAQ5™,™can come out of solution when frozen and it is difficult(but not impossible)to get it back into solution。
- DRAQ5™,™can be diluted in culture media(e.g.RPMI1640)and physiological buffers(egPBS,Hanks’s,etc.)and mixed with fixatives such as formaldehyde。
You can find much more technical information in the folders below:-