enzyme activity

The ability of enzymes to catalyze certain chemical reactions

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synonym enzymatic activity (Enzyme activity) generally refers to enzyme activity

Enzyme activity, also known as enzyme activity, refers to Enzyme catalysis certain chemical reaction Ability. The enzyme activity can be expressed by the conversion rate of a certain chemical reaction catalyzed by it under certain conditions, that is, the faster the conversion rate catalyzed by the enzyme, enzyme The higher the vitality of; On the contrary, the slower the rate, the lower the enzyme activity. Therefore, the determination of enzyme activity is the determination of enzymatic conversion rate. The enzyme conversion rate can be calculated by substrate Of a reduction or product Increase To represent. Enzyme activity can be measured by quantitative measurement Enzyme reaction The amount of product or substrate of reaction time The change of a certain property in the enzyme reaction substrate, such as viscosity change, can also be measured quantitatively. Usually in the enzyme Optimum pH Values and ionic strength And determine the enzyme activity at the specified temperature.^[1]

Chinese name: enzyme activity
Foreign name: enzyme activity
Alias: enzymatic activity

Definition: The ability of enzymes to catalyze certain chemical reactions
Role: Determination of enzymatic conversion rate
Determination method: Determination of initial speed of enzymatic reaction

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definition

finger Enzyme catalysis The ability to react chemically.

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Under the optimal conditions, 1 minute of conversion mole The amount of enzyme required for substrate is one enzyme Dynamic unit (U)。

Specific activity

Per milligram Enzyme protein The enzyme activity. The unit is u/mg. Specific activity The higher the enzyme, the purer the enzyme.

Conversion number

Per molecule enzyme or per Enzyme active center Substrate that can catalyze in unit time Molecular number (TN)。 amount to Enzyme reaction Of Velocity constant kp。 also known as Catalytic constant （Kcat）。 1/kp is called Catalytic cycle 。 carbonic anhydrase Is known Conversion number One of the highest enzymes, up to 36 × 106 per minute, with a catalytic cycle of 1.7 microseconds.

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Generally measured Enzymatic reaction Initial velocity Because there are fewer interference factors at this time, the speed remains constant. reaction rate The unit of is concentration/unit time, available substrate reduction or product It is expressed by the increased quantity. Because the concentration of the product changes greatly from scratch, and the substrate is often excessive, the change is not easy to measure accurately, so the product is often used for determination.^[2]

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