产品回顾
The Science
CyTRAK Orange™can be used asa LIVE or fixed cell counterstain or as a cell location dye to define the perimeter of the cell in a variety of cell-based assays。 It can be excited at488nm and 534nm with optimal emission at615nm。 It can be used in flow cytometry to separate(gate)nucleated cells without the time-consuming and potentially damaging RBC lysis procedures。 It is high ly compatible with existing protocols and proven across a wide range of instrumentation platforms:flow cytometers,epifluorescence and confocal microscopes and image-based HCS platforms。
Why CyTRAK Orange™?
CyTRAK Orange™co-excites at 488nm with eGFP, FITC and Cy2flurophores avoiding the need for laser alignment in confocal instruments when this combination is used。 CyTRAK Orange™has an emission profile that permits the simultaneous acquisition of commonly used“green”fluorphore signals(e.g.eGFP,FITC and Cy2)AND far-red FRET dyes(e.g.PE-and APC-Cy7)combined with discrimination of both the nuclear and cytoplas mic compartments。 A separate series of parameters can be detected with red-exciting fluorphores such as APC,APC-Cy7since CyTRAK Orange™is not excited at633or647nm。 Wide platform compatibility-blue/green excited(Exmax510nm) GFP/FITC compatible-orange emission(Emmax615nm) 多colour compatible-no UV/red excitation Compartments:1 dye-differential nucl:cyto staining Demarks cell boundaries-even staining of the cytoplasm 全负荷开发-工作现场现场直播或fixed cells Time-saving-rapid staining,no-lyse/no-wash protocols Convenient-ready-to-use and buffer compatible 可靠、可靠、可靠、可靠
技术信息
CyTRAK Orange™does not crosslink with or esterify cytoplasmic proteins。 CyTRAK Orange™does the work of two fluorophores。 This leaves more opportunity and choice in designing the assay for the cells of interest。 Products are shipped at ambient temperature,but on receipt packs should be stored at2-8°C。 Do NOT freeze!CyTRAK Orange™can come out of solution when frozen and it is difficult(but not impossible)to get it back into solution。
KEY INFORMATION
KEY REFERENCES
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ATERIAL SAFETY DATA SHEET
CUSTOMER REVIEWS
CyTRAK Orange™is anover orange fluorescent dye from BioStatus。 Be one of the first to review CyTRAK Orange™and publish your experience here。