LysaseA kind ofcatalysisCracked 1.6-diphosphoric acid-D-fructoseGenerate 3-phosphoryl-D-GlyceraldehydeAndα-Dihydroxyacetone phosphoric acidReactive enzyme (at the same timeGluconeogenesisIt can also catalyze the reverse reaction of this reaction), that is, 1.6-Diphosphate D-fructose aldolase (Ec 4.1.2.13).(In a broad sense, it refers to the enzyme that catalyzes the same type of reaction, for example, there are also enzymes that willRhamnosePhosphate aldolase and others are collectively referred to as aldolase).
Aldolase is mainly divided into four dependencies, namely acetaldehyde dependency and pyruvate dependency/PhosphoenolpyruvateDependence, glycine dependence andDihydroxyacetone phosphatedependence.
The reaction is reversibleAldol condensation, △ G °′=5.73 kcal, which is a kind ofGlycolysisIt is an important enzyme.The yeastskeletal muscleMedium purification.come fromyeastThe standard sample of can beα，α′-Dipyridine orCysteineWait for inactivation, with Fe2+、Zn2+、Co2+、Cu2+Activated metal protein, but the enzyme in muscle or plant tissue is not inhibited by bipyridine, etc. In skeletal muscle, the molecular weight of this enzyme is about 150000, and it is composed of four main subunits.
In addition to the above reactions, aldolase also makes various aldehydesDihydroxyacetone phosphoric acidThe effect of condensation.
Two types of aldolase are found in nature.One is inAnimal tissueThe second category is mainly composed ofBacteriaandfungusGenerate.The first kind of aldolase is inhibited by sodium borohydride in the presence of substrate;Type II aldolase coatingEDTAinhibition.[1]
Normal value range
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（1）colorimetry: 5~27U/L for adults, 4 times for newborns and 2 times for children.
(2) Enzyme coupling method: male 2.61~5.71 U/L.Female 1.98~5.54U/L.[2]
Clinical significance
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The determination of serum aldolase is mainly used to diagnose muscle andLiver disease。
（1）Muscle disease：muscular dystrophy、PolymyositisThe activity of serum aldolase was increased in the patients.In muscular dystrophy, the enzyme activity of pseudohypertrophic, limb girdle and distal type is the highest, and that of shoulder brachial type andOphthalmic muscleType A is only slightly elevated or normal.
Generally, aldolase activity decreases with ageMuscular atrophyThe former enzyme activity increased most significantly, so its determination is helpful for the diagnosis of this disease.miocardial infarctionThe activity of serum aldolase in patients increases, generally inChest painIt reached the peak at 24~48 hours after onset, and its change rule was the same as that of AST, but earlier than that of AST.Angina pectoris is normal.
