unit of enzyme

Unit of measure of enzyme activity
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enzyme activity The unit of measure for. 1961 International Conference on Enzymology regulations : 1 enzyme activity unit refers to the optimum condition (25 ℃) condition )Under, 1 μ can be converted within 1 minute mole The amount of enzyme in the substrate, or 1 micromol in the conversion substrate Group The amount of enzyme.
Chinese name
unit of enzyme
Foreign name
U,active unit
Meaning
Measurement of enzyme activity Company
Unit
Enzyme quantity

Company

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U, active unit
Enzyme activity unit: a unit used to express the activity of an enzyme, usually expressed in the amount of enzyme.
Definition of 1961 International Enzymology Conference enzyme activity International unit , which is specified as: under specific conditions, convert 1 μ m within 1 minute mole The substrate, or the amount of enzyme required for 1 micromol of related groups in the substrate, is called an international unit IU of enzyme activity.
In 1972, the international enzymology conference also stipulated an enzyme activity unit, Kat, as the amount of enzyme that can convert 1 mole of substrate in 1 second under the most appropriate conditions. Conversion relationship between Kat and IU: 1 Kat=6 × 10 seven IU, 1IU=about 16.67n Kat.

Specific activity

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Specific activity of enzyme (specific activity): refers to protein The catalytic activity of an enzyme contained in. It is used to measure the purity of enzyme. It is the basic data often used in production and enzymology research.

Conversion number

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Enzymatic Conversion number (Kcat): every active center Or substrates that can be converted by each molecule of enzyme Molecular number Not commonly used in production.

Determination method

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Dynamic method
Enzyme coupling method