(1) Necessary groups in the active center
Including binding group and catalytic group. The binding group has the function of binding enzyme and substrate, which determines the specificity of enzyme; The catalytic group has the function of catalyzing the reaction between enzyme and substrate, and determines the catalytic property of the reaction.
(2) Essential groups outside the active center
It is not directly involved in catalysis, but is necessary to maintain the spatial conformation of the enzyme active center and serve as the binding site of regulators.
Hydroxyl group of serine residue, imidazole group of histidine residue, sulfhydryl group of cysteine residue, carboxyl group of acidic amino acid residue, etc.
(1) Combine with substrate to play a regulatory role
⑵ Participate in the chemical reaction of substrate
⑶ Necessary for maintaining the spatial conformation of enzyme molecule
⑷ Determine the structure of enzyme