Primary structure of protein

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synonym Primary structure (The sequence of amino acids in the protein peptide chain) generally refers to the primary structure of the protein

In each protein Medium, Polypeptide chain in amino acid The order of arrangement of, including Disulfide bond We call it the primary structure of protein, also called primary structure or Basic structure 。 The primary structure of protein is to understand protein structure , mechanism of action and homologous proteins physiological function The necessary foundation of. It refers to all kinds of polypeptide chains Amino acid residue The order in which they are listed.

Chinese name: Primary structure of protein
Foreign name: primary structure

Nature: In protein Amino acid residue Order of
Structure: Amino acid sequence of polypeptide chain

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Primary structure of protein

Primary structure of protein

Refers to the protein Covalent Connected Amino acid residue The order of arrangement of, including Disulfide bond Location of.

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The primary structure of protein includes:

(1) Proteinaceous Polypeptide chain Number of;

(2) Many Peptide chain Amino acid sequence;

Conservative aspartic acid and glutamic acid residues

(3) Within or between polypeptide chains Disulfide bond Number and location of.

determination

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1. Too many determinations Peptide chain Number of

2. Split polypeptide chain

3. Disconnect Polypeptide chain Internal Disulfide bond

4. Determine each Peptide chain Of amino acid composition

5. Identification of N-terminal and C-terminal of polypeptide chain

6. Split the polypeptide chain into smaller peptide segments

7. Determine the amino acid sequence of each peptide

8. Reconstruction of complete polypeptide chain with overlapping peptide Primary structure

9. Determine the position of disulfide bond

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