Amino consists of one nitrogen atom and twohydrogen atomComposition, chemical formula: - NHtwo。In organic chemistry, amino group is the basicBaseMost organic substances containing amino groups have certainalkaliCharacteristics of,
Amino is fundamental in organic chemistryBaseAll organic substances containing amino groups have certainalkaliIt consists of one nitrogen atom and twohydrogen atomComposition, chemical formula - NHtwo。For example, amino acids contain amino acids, which have the characteristics of a certain alkali.Amino is an active and easily oxidizedGroup。In organic synthesis, groups that are easy to be removed are needed for protection.Briefly: 1. Acylation protection, ready to useAcid anhydrideProtection;2. UsebenzylProtection;3、Chiral compoundCBZ, BOC, FMOC and other protective amino acids are commonly used. The amino acid shows positive electricity and is an exclusive electronic group. When amino acids are synthesized into polypeptides, the amino acid can dehydrate and condense with the carboxyl group.
Basic form: (- NHtwo）
Relevant information
Announce
edit
Amino transfer reaction
Experimental principle
Amino transferase, also known as transaminase, can catalyzeα - amino acidAmino group of α - keto acid and α - keto acid-Keto groupExchange, which is called amino exchange.It plays an important role in the intermediate metabolism of protein synthesis and decomposition, and in the interaction and transformation of the metabolism of sugar, fat and protein.When any kind of amino acid undergoes transamination, it is catalyzed by its specific transaminase.Their optimum pH is close to 7.4.In various transaminasesglutamateThe activity of oxaloacetic transaminase (GOT for short) and glutamic pyruvate transaminase (GPT for short) is the strongest.
Phosphate buffer(pH=7.4), 1% glutamic acid solution (neutralized to neutral with KOH), 1% pyruvic acid solution (neutralized to neutral with KOH), 0.1%Potassium bicarbonateSolution, 0.05% iodoacetic acid solution, 15% trichloroacetic acid solution, standard alanine solution (0.1%), standard glutamic acid solution (0.1%), 0.1% hydrationNinhydrinEthanol solutionPhenol solvent.
Operation method
The rabbit was stunned, quickly dissected, and the liver was taken out and cut into pieces under low temperature.Centrifuge the homogenate after weighingsupernatantIs the prepared enzyme solution.In vitro amino transfer reaction.Paper chromatography was used to check 4 points per person (standard alanine solution, standard glutamic acid solution, control supernatant, sample supernatant).Put the sample into the chromatography mask.Use the water solution saturated with phenol. After equilibrium, use a glass tube with a small funnel to add 25ml of saturated phenol reagent from the chromatographic cover into the chromatographic cylinder.Then, immediately cover the plug. When the front edge of the solvent reaches about 2.5cm from the upper end of the filter paper, take out the filter paper, air dry or blow dry to remove the phenol solvent, spray 0.1% ninhydrin ethanol solution, and put the color developing amino acid andNinhydrin reaction, showing purplish red spots on the filter paper.
Key steps and precautions
① Take fresh animal tissues to prepare enzyme solution to prevent enzyme inactivation.
Amino membrane is an important biological transition material. Under chemical action, the amino membrane is activated to immobilize biological molecules.The study of surface modification of materials with amino film is more and more common.This article uses RFMagnetron sputteringThe diamond film was prepared, and the magnetic filtration plasma chemical vapor deposition method was used toDiamond-like carbon filmCarry out surface amino modification to make it have good biological compatibility, so as to expand its application as a biological protection material inBiosensorsApplication in.
Surface modification of diamond-like carbon films
Magnetic filtrationPlasma chemical vapor depositionThe experimental device of the plasma magnetic filter consists of a vacuum system, a discharge system, a plasma magnetic filter system and a peripheral power supply.By adjusting various parameters (volume ratio of nitrogen to hydrogen, excitation current, deposition power), controlDiamond-like carbon filmProcess conditions for amino modification of the surface: excitation current 8 A;Discharge voltage 1.8 kV;Discharge current 2 mA;NtwoAnd HtwoVolume ratio of 1:2, deposition pressure: 30 Pa;Deposition time: 30 min.[2]
