Gene expression vector

Biological terminology

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Construction of gene expression vector (i.e Target gene And Carrier Combination) is implementation genetic engineering The second step is also the core of genetic engineering. Its construction objective Is to enable the target gene to Receptor cell It is stable and can be passed on to the next generation. At the same time, it enables the target gene to express and play a role.

Chinese name: Gene expression vector
Foreign name: gene expression vector

Discipline: biology
Properties: Biological terminology

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Definition and composition

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Genetic engineering

Composition: Promoter 、 Terminator 、 Marker gene 、 Target gene

Gene expression vector

Also known as gene splicing technology and DNA recombination technology , is based on molecular genetics As the theoretical basis molecular biology and microbiology The modern method of DNA molecule , and then import Living cell To change the original genetic characteristics of organisms, obtain new varieties and produce new products. genetic engineering Technology provides a powerful means for the study of gene structure and function.

DNA recombination

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Target gene And Carrier combination

take Target gene And carrier Combined The process is actually the process of recombination of DNA from different sources. If the plasmid is used as the Carrier ，

Gene expression vector

First, use certain Restriction enzyme Cut the plasmid to make a gap in the plasmid Viscous end 。 Then use the same restriction enzyme to cut off the target gene to produce the same sticky end (part Restriction endonuclease Can be cut out Flat end , has the same effect). Insert the cut target gene fragment into the cut of the plasmid complementary base pairing Combined, two Viscous end Fit together, Base Form hydrogen bond between, and then add appropriate amount DNA ligase , catalyzing the formation between two DNA strands Phosphodiester bond So that the adjacent Deoxyribonucleic acid Connect to form a Recombinant DNA Molecules. Humanoid insulin This is how genes interact with Escherichia coli Plasmids in DNA molecule To form recombinant DNA molecules (also called Recombinant plasmid ）Of.

Technical prospect

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The scientific community predicts that the 21st century will be a century of genetic engineering. Genetic engineering is an artificial intervention in biological genetics at the molecular level,

Gene expression vector

Let's start with bioengineering Talking about: bioengineering, also known as biotechnology, is an application of modern life science principles and information and chemical technology Living cell Or its enzymes to process cheap raw materials to varying degrees, providing a comprehensive range of useful products Engineering technology 。 The foundation of bioengineering is Modern life science 、 Technical science And information science. The main product of bioengineering is to provide society with a large number of high-quality fermentation products, such as Biochemical drug , chemical raw materials, energy Biological control As well as food and beverage, it can also provide human with environment treatment, metal extraction clinical diagnosis 、 gene therapy And improved crop varieties Social services 。

Biological engineering mainly includes genetic engineering cell engineering 、 Enzyme engineering 、 Protein Engineering and microbial engineering Wait for 5 parts. Among them, genetic engineering is that people transform biological genes Biological production Special products that people want. along with DNA The secrets of its internal structure and genetic mechanism are presented to people little by little. Biologists are no longer satisfied with exploring and revealing the secrets of biological heredity, but are eager to try to intervene in biology at the molecular level Genetic characteristics 。

Gilbert of America is Base array analytical method The founder of human genome If one of the DNA of a kind of organism Genetic code Fragments can be connected to the DNA chain of another organism, reorganize the DNA, and then we can design new ones according to human wishes genetic material And create new biological types? This is totally different from the traditional practice of breeding biological offspring in the past, which is very similar to the technical science engineering design That is to "construct" and "assemble" this "gene" of this creature and that "gene" of that creature into new ones according to human needs Gene combination And create new creatures. This is completely in accordance with human wishes, from reassembling genes to Neobiosis Generated Biological Science and Technology Is called "genetic engineering", or“ genetic engineering ”

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