Chromatographic method for separation using ion exchanger as stationary phase
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Ion Exchange Chromatography (IEC for short) is based onIon exchangerAs a stationary phase, it is a chromatographic method for separation according to the difference in the binding force between the component ions in the mobile phase and the balance ions on the exchanger when they are reversibly exchanged.Common ion exchangers are:Ion-exchange cellulose, ion exchange glucan andion exchange resin。
Chinese name
Ion exchange chromatography
Foreign name
Ion Exchange Chromatography
Type
A chromatographic method for separation
brief introduction
In ion exchange chromatography, the matrix is composed of charged resin or cellulose.With negative chargeAnion exchange resin;With positive chargeCationic resin。Ion exchange chromatography can also be used for the separation and purification of proteins.Since proteins also have isoelectric points, when proteins are in differentpHUnder the condition, its electrification condition is also different.anion exchangeThe matrix binds negatively charged proteins, so these proteins are left on the column, and then the proteins adsorbed on the column are eluted by improving the salt concentration in the eluent and other measures.
