The gelatinous solid medium is made into a plane shape
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Plate culture refers to the method of making agar or gelatin and other gelatinous solid culture medium into a plane, and then inoculating the cells, tissues or organs of microorganisms or multicellular organisms on the plane, and cultivating them.Plate culture usually uses culture dishes to hold the culture medium.Plate culture is widely used inSoil microbiologyIn the research, it is often used to isolate soil microorganisms to achieve pure culture, and often combined with gradient dilution method to detect the number of colonies in soil samples;It is also often used to observe the shape and type of colonies (mainly bacteria and fungi), conduct antagonistic tests, and determine the potency of antibiotics;In genetic research, it is widely used for tissue culture, embryo culture and pollen culture.[1]
Chinese name
Plate culture
Foreign name
plate culture
Purpose
Observe colony shape and type
Part of speech
noun
Plate culture, plating
This culture is called plate culture.Plate culture is usually carried out in Petri dishes.In bacteriological research, this method is usually used to observe the shape and type of colonies;It can be combined with dilution culture method to isolate certain microorganisms for pure culture (it can be mixed with a small amount of properly diluted bacterial suspension for plane culture, or it can be dipped with a inoculum needle to dip the bacterial suspension in thePlate culture mediumOverline culture);Detection of colony number;Determine the titer of antibiotics.This method is widely used in tissue culture, embryo culture and pollen culture in genetic research.
