Science and Technology Daily, Beijing, May 22 (Reporter Zhang Mengran) - Waseda University of Japan has developed a hybrid nanotube (HyNT) imprinting system for intracellular protein delivery in cooperation with the Japanese Institute of Physics and Chemistry. This innovative technology can simultaneously deliver multiple loads directly to adhesive cells for cancer treatment. The research paper was published in the latest issue of Analytical Chemistry.
Intracellular protein delivery brings hope for the development of safer, more targeted and more effective therapies. This new system can effectively eradicate cancer cells and minimize the impact on healthy cells by transferring lactate oxidase (LOx) and ubiquitin (UQ) proteins. This not only provides a broad prospect for precise cancer treatment, but also ensures the vitality of normal cells.
The researchers briefly explained the assembly of the imprinting system. HyNT can be synthesized on the gold nanotube film, and then assembled with the glass tube to form the imprint that can physically insert HyNT into the cell.
The researchers then explored the potential of delivering LOx for cancer treatment. Through the innovative imprinting system, they successfully transferred LOx to healthy mesenchymal stem cells (MSCs) and cancerous HeLa cells. MSC cells were not affected, but cancer cells showed significant death, and their vitality decreased over time.
The results showed that the imprinting system achieved 89.9% delivery efficiency and 97.1% cell survival rate when delivering the target protein, highlighting the system's ability to maintain the health and integrity of processing cells. This method is effective in selectively targeting and killing cancer cells, while not "harming innocent people", providing a good strategy for cancer treatment.
Researchers said that in addition to medicine, HyNT imprinting system can also be used in agriculture and food industry, which is expected to promote crop production and food development. With accurate cell manipulation and efficient delivery, HyNT imprinting system is expected to change biomedical research, clinical practice and other fields, pave the way for personalized intervention, and shape the future of modern medicine.
