Half inhibition concentration (or half inhibition rate), namely IC50.In indirect competition ELISAstandard curve The standard curve is an S-shaped curve.
In ICELISA, the OD value of the control hole without inhibitor is B0, the OD value of the hole with inhibitor added is B, and B/B0 is called the binding rate. When the binding rate is 50%, the corresponding concentration of inhibitor is called IC50.Generally, the smaller the IC50 value is, the stronger the specificity of the antibody is.
minimumDetection limitIt is the minimum concentration of the standard curve of the kit, which is less than the minimum concentration or greater than the maximum concentration, that is, the concentration that is not within the curve range isinaccurate, can be diluted if it is greater than the maximum concentrationPosttest。
IC50 is the 50% inhibition concentration, that is, the corresponding concentration when B/B0=50%. The half inhibition is used to measure the sensitivity of the antibody. The lower the half inhibition, the higher the sensitivity of the antibody.
Generally speaking,KitThe maximum and minimum range should be the antibody used to detectStandardThe detection limit is divided into the maximum and minimum detection limits.
The lower detection limit is generally the theoretical value, which is based onstandard curve The calculated minimum value that can be detected theoretically;limit of quantitationIt is the lowest value that can be quantified during actual detection;And the largestDetection limitIt is the drug concentration when the inhibition rate reaches 100% in actual operation.
Lower detection limit（LOD）Corresponding OD value: OD (LOD)=B0-3SD
Limit of quantitation（LOQ）Corresponding OD value: OD (LOQ)=B0-10SD
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IC50，the half maximal inhibitory concentration，represents the concentration of an inhibitor that is required for 50% inhibition of things like an enzyme，a cell，a cell receptor or amicroorganism.
It's commonly used as a measure of drug effectiveness. Another measure of drug efficacy isEC50which represents the concentration of a compound that is required to obtain 50% of the maximum effect. Ex. EC100 would be the minimum concentration of a compound to obtain 100% of its effect.
