The sticky end is cut by restriction enzymeDNADouble chainIn many cases, the incision is not flush, but achain lengthFor example, after AAAAAAAAAATTTTTTTTTT is cut open, it may become: AAAAAAAAAATTTTTTTTTTTT such ends can be combined through base complementation, as if they are sticky.
But if the cut end is flush, it is not sticky.In addition, use differentEnzyme digestionEven if the ends of the two sections are not flush, the parts on both sides are not sticky if they cannot complement each other.
Chinese name
Viscous end
Foreign name
Sticky Ends
Field
DNA recombination
Related concepts
Flat Ends
Definition
DNA double strand cut by restriction enzyme
definition
Two single strand cuts of DNA cut by restriction enzyme, with several protrudingnucleotide, they are just complementary and matched, such a cut is called viscous end.