trichoderma reesei

Trichoderma

filamentous fungi Trichoderma reesei is multicellular Of Eukaryotic microorganism , the asexual form of Hypocrea jecorina, belonging to Arbuscularia （Moniliales） Trichoderma （Trichoderma）。 As an industrial strain, it is used to produce enzymes that decompose different plant materials, including cellulase 、 Hemicellulase , protease, amylase, etc.

Latin name: Trichoderma reesei
circles: Fungal kingdom

order: Arbuscularia
genus: Trichoderma
Chinese scientific name: trichoderma reesei

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Colony characteristics

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The colony is in the form of widely spread cotton wool. At first, it is white, dense and flat mycelium, and then the edge appears light green Spore Bundle area, the reverse side is colorless. Conidiophore The short lateral branches of hyphae are transparent and multi branched; Pedicel bottle shaped, curved in the middle; Conidia Oval or long, single cell, transparent, colorless, smooth wall, green when stacked.

gene expression

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A major fungus produced by Trichoderma reesei Cellulase Cellobiohydrolase I, composed of Single copy gene Encoding, its output can reach Trichoderma reesei extracellular Secretory protein 50% of the total [1]. It can be seen that cbh1 Promoter It is a strong promoter. Therefore, in the genetic transformation of Trichoderma reesei, the promoter of cbh1 and Terminator Sequence construction vector, and use cbh1's Precursor peptide Sequence guidance Recombinant protein Secretory expression was performed. Trichoderma reesei has synthetic proteins and Secretory protein Ability; It also has eukaryotic secretion mechanism and probably has protein modification properties similar to mammalian system, such as high mannose Type and N-glycosylation [2] Etc. Due to the above excellent properties of Trichoderma reesei and the mature conditions of its industrial scale fermentation, these have promoted the genetic transformation of Trichoderma reesei, which is homologous or heterologous Secretory protein The emergence of provides an effective way.

functional analysis

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Trichoderma reesei not only has many advantages suitable for protein production, but also has no toxicity to humans and does not produce under the conditions of enzyme production Mycotoxin And antibiotics. In recent years, the practice shows that the recombinant strain of Trichoderma reesei is safe and harmless after genetic engineering surgery.

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