Free acid

chemical substances

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Free acid, also known as 3,3,5,5-tetramethylbenzidine, melting point 168-171 ° C, is mainly used as catalase substrate in ELISA reaction, which can produce soluble light blue end products.

Chinese name: Free acid
Foreign name: TMB

molecular weight: two hundred and forty point three five
EINECS: 259-364-6

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Basic Introduction

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CAS No.: 54827-17-7

Molecular formula: C16H20N2; [-C6H2(CH3)2-4-NH2]2

Alias: 3,3,5,5-tetramethylbenzidine

Alias English name: 3,3,5,5-Tetramethylbenzidine

Characteristics

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TMB as catalase Substrate is used in ELISA reaction, which can produce soluble light blue end products and can be used at 370 or 620-650 nm Spectrophotometer Read. The TMB reaction can be terminated with 2 M H2SO4 (producing yellow) and can be read at 450 nm. Therefore, TMB is used to measure blood, heme and Peroxidase Sensitive and specific reagents.

Melting point: 168-171 ° C

Storage mode

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Sealed storage

purpose

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TMB has gradually replaced the strong carcinogen benzidine and other carcinogenic benzidine derivatives, and has been used in clinical tests, forensic inspection, criminal detection, environmental monitoring and other fields. Especially in clinical biochemical tests, TMB acts as Peroxidase The new substrate of Immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) have been widely used.

（1） The main application projects of TMB are as follows:

1. Fingerprint detection is the most effective way to show occult blood fingerprints on public security

2. Rapid detection of alcohol in saliva

3. Preparation of urine test paper

4. Hepatitis virus detection

5. Pregnancy test

6. Glucose in blood and urine hemoglobin Rapid determination of albumin

seven Fecal occult blood test

8. Determination of granulocyte value in blood

9. Detection of steroids and sex hormones

10. Determination of enzyme activity

11. Analysis and detection of antigen, antibody and genetic material