Mixed functional oxidase, also called multifunctional oxidase, exists in microsomes of human and animal liver and lung cells[2]。
brief introduction
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The enzyme system mainly exists in the liver and other tissues, but only in a small amount.Mixed functional oxidase is not a single enzyme, but a group of enzymes.When the cell homogenate is ultracentrifuged, it can be found that this enzyme mainly exists in the microsomal components of cells.When it is forcibly separated from the endoplasmic reticulum membrane to which it is connected, the enzyme will lose its activity, so there is no good way to purify it[3]。
form
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Mixed functional oxidase system includesCytochromeP-450, NADPH cytochrome P-450 reductase, cytochrome B-5NADH-cytochrome B-5 reductase, aromatase, epoxide hydratase, phospholipid, etc.Among them, cytochrome P-450 is the most important. It forms a "reactive oxygen" complex with molecular oxygen, which can oxidize exogenous chemicals into human liver and lung[1]。
Metabolic reaction
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Through its oxidative metabolism, two reactions can be generated: one is the degradation reaction, which can make the original chemical substances become low toxic or non-toxic substances to be discharged from the body.The second is the activation reaction, which can transform the original chemical substances into electrophilic substances, leading to increased toxicity and becoming mutagens or ultimate carcinogens.
significance
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The in-depth study of the interaction between mixed functional oxidase system and exogenous chemicals is of great significance for further understanding the toxic effects of exogenous chemicals at the level of molecular biology[1]。
