chromatographicThe substance carrying the components to be measured forward in the process is called mobile phase.Andstationary phasebe inEquilibrium stateAnother phase that drives the sample forward.
chromatographicThe substance carrying the components to be measured forward in the process is called mobile phase.Andstationary phasebe inEquilibrium stateAnother phase that drives the sample forward.The substances used as mobile phase are:Gas, liquidSupercritical fluidEtc.Common mobile phases mainly include:acetonitrile-aqueous solution, acetonitrile acetic acid aqueous solution, methanol aqueous solution, acetonitrile phosphoric acid aqueous solution, etc[1]。
characteristic
Announce
edit
Liquid chromatography is the separation of sample components through mass exchange between column packing and mobile phase. Therefore, mobile phase is required to have the following characteristics:
(1) The mobile phase has a certain solubility to the sample to ensure that the sample components will not precipitate in the column (or remain in the column for a long time).
(2) The mobile phase does not react with the sample
(3) The viscosity of mobile phase should be as small as possible to obtain good separation effect;Reduce the column pressure drop and extend the service life of the pump (the viscosity of mobile phase can be reduced by increasing the temperature).
(4) The physicochemical properties of the mobile phase should be compatible with the detector used.If UV detector is used, it is better to use solvent with low UV absorption.
(5) The boiling point of the mobile phase should not be too low, or bubbles will easily occur, which will lead to the failure of the experiment.
(6) After the mobile phase is prepared, degassing must be carried out.The removal of trace gases dissolved in the mobile phase is not only conducive to detection, but also can prevent the trace oxygen in the mobile phase from interacting with the sample[2]。
