Oil immersion objective

Objective lens that drops a drop of oil on the slice before observation

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Oil immersed objective is to drop a drop of oil on the slice before observation objective lense , because the optical properties of the oil Glass Almost, no refraction , objective lens resolving power Substantial increase.

Chinese name: Oil immersion objective
Interpretation: Drop a drop of oil on the slice before observation objective lense

Expand: In addition to oil immersed objective lens (black circle), there is also water immersion objective lense , objective lens dipped in glycerin, etc.

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brief introduction

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Oil immersed objective is to drop a drop of oil on the slice before observation objective lense 。 According to Abbe formula R=0.61 λ/N.A, the greater the aperture ratio, the better the resolution; By N A=n · sin α/2, it can be seen that the larger the refractive index n, the higher the aperture index. Oil can improve the refractive index of the medium between the objective lens and the specimen, so oil immersion objective lens can improve the microscope resolution.

Use of oil immersion mirror

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Observe with an oil immersion lens, find the sample area to be observed under a high-power or low-power lens, and use the coarse focusing button to lower it first Stage , and then Oil mirror Go to the working position. Add a drop of cedar oil to the sample area to be observed, watch from the side, and carefully raise the stage with the coarse adjustment button, so that the immersion mirror is immersed in cedar oil and almost connected with the specimen piece. take Condenser Raise to the highest position and fully open aperture 。 Slowly lower the stage until a clear image appears in the field of vision, carefully observe and record.^[1]

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In addition to oil immersed objective lens (black circle), there is also water immersion objective lense , objective lens dipped in glycerin, etc.

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