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BiomacromoleculeUnder certain pH conditions, it is usually charged, and when it is placed in an electric field, it will move towards thechargeFor electrode migration with opposite properties, the migration rate is called electrophoresis rate.
Under certain pH conditions, biological macromolecules are usually charged. When they are placed in an electric field, they will migrate to the electrode with opposite charge properties at a certain speed. The migration speed is called electrophoresis rate.[1]
Under physiological conditions,DNAThe phosphate group in the molecular sugar phosphate skeleton is ionized, so DNA is actually polyanionState, moving to the positive direction in the electric field.
Sugar-phosphoric acidThe skeleton is structurally repeated, so an equal amount of double stranded DNA carries almost an equal amount of net charge.
If the electric field intensity is constant and the electrophoresis medium is the same, the electrophoresis rate depends onnucleic acidMolecular size and configuration.Molecules with similar configurations: the larger the molecular weight, the slower the migration.
Molecules with the same molecular weight (such as plasmid):
cccDNAThe migration is the fastest, followed by L-DNA, and ocDNA is the slowest.
