(4) Change the nature of the mutant enzyme to make it insensitive to antibiotics;
(5) ChangeMetabolic pathwayEtc.Drug resistance mutations can occur not only in nuclear chromosome genes, but also inplasmidOn.stayShigella dysenteriae(Shi; ellu d: seuter: ue)EnterobacteriaceaeThere are often drug resistant particles (i.e. R factor or R plasmid), in which there can be multiple antibiotic resistant genes, and this plasmid will transfer between different strains or strains, which is very harmful to the prevention and treatment of infectious diseases.In microbial genetic research and breeding[1]Biotin resistance is an ideal marker for strain screening and genetic recombination.
In June 2013,britainprime ministerCameronAnnounce that it will“Longitude Award”In 2014, 300 years after the award, this challenge event was launched again[5]，Solicit all the people in the world to solve the century's problem - the method of antibiotic resistance was selected“Longitude Award”[6]，The "reward order" amounts to 10 million pounds (about 16.9 million dollars)[7]。
