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Initiator is the synthetase of primer RNA in DNA replication. In the narrow sense, initiator refers toEscherichia coliThe product of dnaG genetic factor.
DnaG genetic factorThe product ofprotein, YesEscherichia coliAnd Escherichia colihostThe DNA replication of many bacteriophages is necessary.By using φ × 174,G4 bacteriophageThe DNA of isTemplateOfIn vitroThe analysis of replication lines can also determine the structure of RNA synthesized by the initiator enzyme.E. coliT-series bacteriophageOn the other hand, the inherent genetic factors of bacteriophages corresponding to the function of dnaG can be detected.
At the beginning of replication, there must be a segment of RNA asprimer, this primer is not separated from the template after synthesis, buthydrogen bondCombined with formwork.This primer is composed of a uniqueRNA polymeraseInitiate enzyme synthesis.In Escherichia coli, this enzyme isSingle chain, molecular weight 60 000, eachCellsThere are 50-100 molecules in.They are made by dnaGgeneCoded.This enzyme is quite inactive when it exists alone, and only combines with relevant proteins to form acompoundOnly whenactivity。[1]
