Escherichia coli bacteriophage refers to a kind of bacteriophage parasitic in Escherichia coli, belonging to a kind of cell virus.Once it leaveshost cell, you can't copy and grow.
Escherichia coli bacteriophage refers to a kind of bacteriophage parasitic in Escherichia coli, belonging to a kind of cell virus.Another characteristic of bacteriophage is its specificity. One kind of bacteriophage only lives in one kind of bacteria, but cannot live in another kind of bacteria. Human bacteriophage is a kind of Escherichia coliTemperate bacteriophageIt is an excellent carrier molecule in genetic engineering, which is used as a porter to carry animal genes into E. coli cells.
Reproductive characteristics
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Escherichia coli bacteriophage
The reproduction process can be divided into four steps: adsorption, invasion, replication and synthesis, and release.
(1) Adsorption.Phage is highly specific for its tail end to adsorb to sensitivecell surfaceA specific site on a cell wall, such as a flagellum, or cilia.
(2) Intrusion.Inject your own genetic material (DNA)host cellInside.
(3) Copy and Compose.It refers to the replication of bacteriophage DNA and protein coat.After phage DNA enters the host cell, it immediately causes the metabolic changes of the host, and the nucleic acid in the host cell cannot replicate andSynthetic proteinAnd controlled by the genetic information carried by bacteriophage nucleic acid, the nucleic acid is copied by the host cell's synthesis mechanism and enzyme, and then the bacteriophage protein is synthesized.Nucleic acid and protein are assembled to synthesize new phages.
(4) Release.When the phage particles are mature, the host cells are hydrolyzed by the phage hydrolase, and the host cells are lysed, and the phage is released.
