Method of connecting reactants to an insoluble solid carrier
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Solid phase synthesis is a synthesis method that connects reactants on an insoluble solid carrier.It is easy to see that the procedure of lengthening peptide chain is not only complicated, but also the final yield is not high due to multiple separation and purification.The solid phase synthesis method proposed in the early 1960s avoided some previous shortcomings.When solid phase synthesis is carried out, the solid carrier (such asPolystyrene resin）Chloromethyl (- CH2Cl) or other groups that can react with carboxyl groups are introduced to react with amino acid protected, and the first amino acid is immobilized on the resin.[1]
Chinese name
Solid state synthesis
Foreign name
solid phase synthesis
Features
Simplifies and accelerates multi-step synthesis
Benefits
Avoid losses due to manual operation and material transfer
Because the reaction can be carried out in a simple reactor;The covalently linked peptide chain of the solid phase carrier is in an appropriate physical state, which can be quickly filtered and washed without completing the intermediate purification, avoiding the lengthy recrystallization or column separation steps in the liquid phase peptide synthesis, and avoiding a lot of losses in the separation and purification of the intermediate;Use excessive reactants to force individual reactions to complete, so as to obtain high yield of the final product;Increase solvation and reduce intermediate product focusing;The peptide chain on the solid phase carrier and the slightly cross-linked polymer chain are closely mixed, which produces a mutual solvent effect, which is unfavorable to the thermodynamics of peptide self aggregation and suitable for the reaction.The main problem of solid phase synthesis is that the intermediate heteropeptides on the solid phase carrier cannot be separated, so the purity of the final product is not as good as that of the liquid phase compound, and it must be purified by reliable separation methods.
