Subunit refers to a part of a whole, but it is also an important component of the whole. These parts cannot be directly controlled, but need to be indirectly controlled by the main body. Such main components, called subunits (or subunits), are mostly used for protein structure analysis and application[1-2]。
for exampleRibosome subunityesribosomeIt is composed of large and small subunits.becauseSettlement coefficientDifferent ribosomes can be divided into 70S type and 80S type.70S ribosomes mainly exist inProkaryotic cellAnd chloroplastsMitochondrial matrixMedium, its small subunit is 30S, and its large subunit is 50S;Type 80S ribosomes mainly exist in the cytoplasm of eukaryotic cells, with a small subunit of 40S and a large subunit of 60S[1][3]。
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70S ribosomeSubunits and mRNAtRNAcompoundcrystal structureThe resolution of 3.1-3.3 angstrom is achieved in the presence and absence of paramycin.The binding of tRNA induces the movement of the entire domain of the 30S subunit and changes in the conservative configuration, especially A1492, A1493, and 16SRNAG530.These bases interact closely with the first two base pairs of codon anti codon.Therefore, similar annexation tRNAs can be distinguished by recognizing base pairing rules.As for the third locus, the swing position, can freely adapt to this non classicalBase pairing。Paromomycin promotes the binding of similar annexation tRNAs by partially inducing these structural changes.
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Ribosomal proteinpreparationPolyclonal antibodyThe isolated rat liver ribosome subunits were studied by sandwich negative staining under electron microscope.The results showed that there were seven ribosomal proteins on the surface of small subunits of rat liver ribosomes, three of which (S13/16, S19, S24) were reported for the first time.Six of the seven proteins are located on the outer surface of the 40S ribosomal subunit, and only one (S24) is located on the inner surface.Based on previous research results, it has been located on the surface of 40S ribosome
【DOI】：cnki:ISSN:1671-0770.0.1990-02-088
G. Lutsehetal In this paper, polyclonal antibodies were prepared from nucleosomes, and three of them (513/16, 519, 524) were reported for the first time.Six of the seven proteins are located at 405Ribosome subunitThe outer surface of the.
Sequenced as clonednosema bombycis (Zhenjiang strain, Nosemabombycis), using the ribosomal small subunit RNA (SSUrRNA) coding gene (1205bp) as the template, and using the probe labeled by the random primer synthesis methodRestriction endonucleaseEnzymatic digestion products ofSouthern hybridizationIn the atlas.
Other protein subunits (subunits)
Many proteins with complex functions are complexes composed of different subunits. For details, please refer to the books on protein structure[2]。
