Cica Geneus®ESBL Genotype Detection KIT2
Extended Spectrumβ-Lactamase:ESBL is an enzyme hydolyzes wideβ-lactam
Extended Spectrumβ-Lactamases（ESBLs）are enzymes that mediate resistance to
penicillins，extended-spectrum third generation cephalosporins and monobactams。
This ESBLs-producing bacteria has important clinical and therapeutic implications
Therefore，the spread of these pathogens puts in peril our antibiotic options。
This kit can detect nine representative ESBLs genotypes.This kit was collaborativery
deveroped by Dr.Ikeda，Dr.Kayama，Dr.Ohge and Dr.Sugai at Hiroshima University。
Characteristics
●This kit can detect representative9genotypes for group of ESBLs。
（TEM，SHV，CTX-M-1，CTX-M-2，CTX-M-8，and CTX-M-9and CTX-M-25，CTX-M chimera，GES（ESBL type）。
●Required time is approximately3hours and suitable foruse in common thermal cycler。
●You can use the same PCR condition asour CicaGeneus®AmpC and Carbapenemase genotype detection kit2。
工作流程
| ① |
Isolate ESBL producing bacteria |
| ② |
DNA提取 |
| ③ |
多功能PCR |
| ④ |
Agarose gel electrophoresis |
| ⑤ |
检测，检测 |
目标genes和its amplicon size
| |
目标gene |
Amplicon size（bp） |
| Reaction mixture.1 |
bla CTX-M-25 group |
522 |
| blaCTX-M chimera |
391 |
| bla CTX-M-1 group |
268 |
| bla CTX-M-8 group |
189 |
| Reaction mixture.2 |
blaSHV |
655 |
blaCTX-M-2 group
|
475 |
| blaCTX-M-9 group |
350 |
| bla GES（ESBL type） |
228 |
| blaTEM |
132 |
Example of the electrophoretic pattern
M:100bp DNA Ladder，P:Reagent E，
1:CTX-M-25,2:CTX-M-64（chimera），3:CTX-M-1，
4:CTX-M-8,5:SHV，6:CTX-M-2,7:CTX-M-9，
8:GES-1（ESBL type），9:TEM，10:GES-2（Carbapenemase type），
N:Negative control（TE Buffer）
Brochure
Cica Geneus ESBL Genotype Detection KIT2
