QX200 AutodG微滴式数字PCR系统

描述

描述

 

QX200 AutodG微滴式数字PCR系统,可提供对靶DNA或RNA分子的绝对定量,兼容基于EvaGreen核酸染料和探针的ddPCR应用。其自动化微滴生成仪简化了ddPCR工作流程,使数字PCR既实用又具扩展性。

AutoDG系统消除了操作人员之间的差异,即不同的使用者和反应板,都能获得更稳定一致的微滴计数,充分领略ddPCR技术的精密度。AutoDG系统还可以更大限度地减少人工操作的时间——加载样本所需时间不到5分钟,并且在微滴生成过程中无需监视或人工干预。

QX200 AutodG微滴式数字PCR系统的功能和优势

自动化微滴发生器—简化了ddPCR工作流程并提供了灵活性:

  • 45分不到的时间内可产生96次ddPCR反应的微滴
  • 与QX100和QX200微滴式数字PCR系统大家兼容
  • 一台AutoDG可为4-5台微滴阅读器持续制备微滴
  • 通过大型彩色触摸屏界面和LED指示灯指导装置和安装
  • 用户可按8孔增量自定微孔板布局,提供了每次运行之间的灵动性
  • HEPA过滤的外壳减少了污染的可能性,并允许系统放置在任何实验室环境中

微滴数字PCR技术—针对各种应用的更精确、更灵敏的数字PCR解决方案,可提供:

  • 灵活的数字PCR化学-TaqMan水解探针和EvaGreen分析进行了优化
  • 纳升微滴分配-减少扩张效率差和PCR抑制剂引起的偏差
  • 便捷的检测设计—无需标准曲线

QX200微滴式数字PCR系统的应用

  • 癌症生物标志物研究和拷贝数変异—测量不同程度的癌症突变,检测稀有标DNA拷贝,并以变色的灵敏度和分辨率解析拷贝数变异状态。
  • 病原体检测—利用QX200系统极高的精密度量化病原体检测和监测中目标DNA或RNA分子的小倍数变化
  • 次世代测定序—无需使用标准曲线即可对NGS文库进行绝对定量
  • 基本要素分析—可实现低丰度mRNA和miRNA的小倍数变化进行可依靠且可重复的测量
  • 环境监视—测试各种环境样品,如土壤和水
  • 食品検测-使用经验证的ddPCR方法对转基因生物(GMO)进行常规评价

所需仪器

QX200 AutodG微滴式数字PCR系统需要使用带96-深孔反应模块的C1000Touch热循环仪和式PX1PCR反应板热封仪

所需耗材

ddPCR试料,用于EvaGreen或TaqMan水解探针的应用

More Information

下载微滴式数字PCR应用指南详细了解各种应用类型的实验设置信息,包括:

  • Droplet Digital PCR applications guide
    • 拷贝数変化分析
    • 稀有突变和序列检测
    • 基本要素表
    • 次世代测序文库分析

 

QX200微滴式数字PCR系统的性能数据

 

我々の成功事例视频,了解更多关于微滴式数字PCR系统在各种研究领域的应用信息。

浏览我们的微滴数字PCR出版物,内有科学期刊文章的链接、会议海报、BioRadiations文章和Bio-Rad技术报告。

我々の访问应用&技术网页,了解更多相关微滴数字PCR技术数字PCR工作流程的情报。

技术指标

技术指标

起始样品量,µl
20
自动化微滴生成器载量
1-96样/运行
微滴/20µl样品
20,000
QX200™微滴读取器载量
1-96样
照明、灯具
发光二极管
样本检测
多光子计数器
检测通道
FAM (EvaGreen), HEX (VIC)
Linear dynamic range
5 orders of magnitude
精度
±10%
各96孔板的微滴数,百万
~1.5
自动化微滴生成器尺寸(宽x深x高)
打开:66x56x89cm(26x22x35in)
QX200微滴读取器尺寸(宽x深x高)
66 x 52 x 29 cm (26 x 20 x 11 in)

注文

注文

items

Use the filters below to refine results!

Image
qx200-auto-dg-droplet-digital-pcr-system-186-4100-thumb.jpg
1864100

QX200 AutoDG Droplet Digital PCR System enables and automates up to 2-color multiplexing for up to 96-samples per run providing clear discrimination of multiple targets. System includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QX Manager Software, Standard Edition.

Image
qx200-droplet-reader-186-4003-thumb.jpg
(停产) QX200 Droplet Reader
1864003

Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System

配套设备

配套设备

Image
PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(停产) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

试薬&试薬箱

试薬&试薬箱

Image
qx100-droplet-pcr-supermix-2ml-186-3023-thumb.jpg
1863023

2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems

Image
qx100-droplet-pcr-supermix-5ml-186-3024-thumb.jpg
1863024

5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems

Image
qx100-droplet-pcr-supermix-25ml-186-3025-thumb.jpg
1863025

25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems

Image
qx100-ddpcr-supermix-probes-2ml-186-3026-thumb.jpg
1863026

2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems

Image
qx100-ddpcr-supermix-probes-5ml-186-3010-tn.jpg
1863010

5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems

Image
qx100-ddpcr-supermix-probes-25ml-186-3027-tn.jpg
1863027

25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems

Image
qx100-ddpcr-supermix-probes-50ml-186-3028-thumb.jpg
1863028

50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital™ PCR Systems

Image
qx200-ddpcr-evagreen-supermix-186-4033-thumb.jpg
1864033

200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System

Image
qx200-evagreen-ddpcr-supermix-186-4034-thumb.jpg
1864034

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System

Image
qx200-evagreen-ddpcr-supermix-186-4035-thumb.jpg
1864035

2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System

Image
qx200-evagreen-ddpcr-supermix-186-4036-thumb.jpg
1864036

5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX600/QX200 Droplet Digital™ PCR System

Image
qx100-ddpcr-buffer-control-kit-186-3052-thumb.jpg
1864052
9 ml (2 x 4.5 ml), 2x buffer, for use as a blank control with Droplet Generation Oil for EvaGreen
Image
qx100-ddpcr-buffer-control-kit-186-3052-thumb.jpg
1863052
9 ml (2 x 4.5 ml), 2x buffer, for use as blank control with Droplet Generation Oil for Probes
Image
ddpcr-library-quantification-kit-for-ilumina-truseq-186-3040-thumb.jpg
1863040

200 x 20 µl reactions, includes ddPCR Library Quantification Assay and ddPCR Supermix for Probes (No dUTP), for quantification of Illumina TruSeq libraries using the QX200™/QX100ddPCR™ Systems

耗材

耗材

Image
pierceable-foil-heat-seal-181-4040-thumb.jpg
1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer
Image
qx100-droplet-reader-oil-2l-186-3004-thumb.jpg
1863004

2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems

関连资料

関连资料

Number 描述 Options
3600
ddPCR Family Brochure
3600
ddPCR Family Brochure
6718
Guidelines for Phasing Variants Using Bio-Rad's Droplet Digital PCR System Application Note
6561
QX200 AutoDG Droplet Digital PCR System Flier
[ Add to Cart (Free) ]
10043138
Instruction Manual, Automated Droplet Generator for Droplet Digital™ PCR Systems
10000107223
QX200 Droplet Reader and QX Manager Software Standard Edition User Guide
10000118144
QX Developer Software, User Guide
10028376
QX200™ ddPCREvaGreen Supermix Product Insert
10048259
ddPCR Supermix for Residual DNA Quantification Product Insert
6782
ddPCR™ Multiplex Mutation Screening Kits Flier, Ver D
[ Add to Cart (Free) ]
6721
ddPCR™ Supermix for Residual DNA Quantification Flier, Rev A
6712
Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital™ PCR Application Note, Ver B
7204
Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies, Ver A
7271
Current Challenges and Future Impact of Cellular and Genetic Immunotherapies for Human Health
3530
A Comparability Study of the C1000 Touch 96-Deep Well and PTC Tempo Deepwell Thermal Cyclers for Use in Droplet Digital™ PCR Workflow