Binding of Biotinylated Human PD-1 to Immobilized Human PD-L1 in a Functional ELISA Assay Immobilized human PD-L1 protein at2μg/mL（100μL/well）can bind human PD-1-Biotin with a linear range of 0.01-0.2μg/mL when detected by Streptavidin-HRP。Background was subtracted from data points before curve fitting。（QC tested）
Inhibition of PD-1-PD-L1binding by Anti-PD-1Neutralizing Antibody measured using the PD-1[Biotinylated]：PD-L1Inhibitor Screening ELISA Assay Pair（Cat.No.EP-101） Anti-PD-1neutralizing antibody was diluted from10μg/mL to 0.078μg/mL（69.628nM to 0.544nM）and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin。Assay was performed according to the protocol in PD-1[Biotinylated]：PD-L1Inhibitor Screening ELISA Assay Pair。Background was subtracted from data points prior to log transformation and curve fitting。（QC tested）
组件
产品名称
大小，大小
高bind Plate
1plate
Human PD-L1
25μg
Anti-PD-1 Neutralizing Antibody
20μg
Human PD-1-Biotin
5μg
Streptavidin-HRP
10μg
组合缓冲器
12 mL
10×Washing Buffer
50 mL
Blocking Buffer
50 mL
Substrate Solution
12 mL
停止解决方案
7mL
Bio X Cell公司
In Vivo提供用于测定的大容量单克隆抗体
Bio X Cell公司以毫克到克单位的大容量制造·销售高品质的单克隆抗体。因为是高纯度且低内毒素浓度，没有添加保存剂或稳定剂in vivo是最适合研究的产品。
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产品编号：SEA789HU
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![Inhibition of PD-1-PD-L1binding by Anti-PD-1Neutralizing Antibody measured using the PD-1[Biotinylated]：PD-L1Inhibitor Screening ELISA Assay Pair（Cat.No.EP-101）](/files/EP-101-E2.jpg)
