| 熊鋼，王曉清，王佩，陳貞年，周先文，康驪，曾志南.泥東風螺EST-SSR開發及其群體遺傳多樣性分析.漁業科學進展，2020,41（4）：117-124 |
| 泥東風螺EST-SSR開發及其群體遺傳多樣性分析 |
| Development and Genetic Diversity Analysis of Babylonia lutosa with EST-SSR Markers |
| 投稿時間：2019-04-17 修訂日期：2019-05-12 |
| DOI： |
| 中文關鍵字： 泥東風螺 轉錄組 EST-SSR 遺傳多樣性 |
| 英文關鍵字： Babylonia lutosa Transcriptome EST-SSR Polymorphism |
| 基金項目： |
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| 摘要點擊次數： 1522 |
| 全文下載次數： 1315 |
| 中文摘要： |
| 本研究採用MISA軟件分析泥東風螺（Babylonia lutosa）轉錄組中微衛星資訊。結果顯示，從轉錄組中共獲得16324個SSR，共有181種重複基元；泥東風螺轉錄組中不同類型微衛星的重複基元具有不同的分佈特徵，其中，二核苷酸重複基元中AC/GT（70.58%）重複基元以重複6次出現頻率占優；長度為12~20 bp的SSR占63.95%，長度為21~25 bp的SSR占9.14%，總體的平均長度為18.4 bp。隨機選取其中50條序列設計引子，通過對福建野生泥東風螺群體（WP）DNA樣本進行PCR擴增和分型，結果獲得23個多態性位點，等位基因數目為2~7個不等，期望雜合度（Ne）為0.190~0.937，觀察雜合度（No）為0.065~0.936，多態性資訊含量（PIC）為0.061~0.777，有4個位點顯著偏離哈迪溫伯格平衡（Hardy-Weinberg equilibrium，HWE）（P<0.05）。對野生群體和養殖群體（BP）遺傳多樣性分析顯示，野生群體和養殖群體的平均Ne分別為0.491和0.544，平均No分別為0.477和0.564，平均PIC分別為0.541和0.407。Fis結果顯示，野生群體和養殖群體分別有13個和9個位點雜合子過剩。群體間遺傳分化指數（Fst）為0.001~0.655，平均值為0.053（0.05 |
| 英文摘要： |
| Babylonia lutosa is a marine shellfish that has high economic values.In recent decades，the natural resource of B. lutosa has declined due to the environment destruction and overfishing.To further understand the level of genetic diversity and population genetic structure of B. lutosa，we have evaluated the information characteristics of B. lutosa’s microsatellites，after obtaining transcriptome sequences using MISA software.The results show that a total of 16342 microsatellites and 181 nucleotide repeat motifs were identified.Different types of repeat microsatellites had considerably different distribution characteristics.Mononucleotide and dinucleotide microsatellite repeating units were the most abundant in the B. lutosa transcriptome，in which 6 repeats of AC/GT（70.58%）were the dominant repeating dinucleotide units.The length of the dominant repeating units was 12~20 bp（63.95%）and 21~25 bp（9.14%），respectively，and the average length was 18.4 bp.Among the 50 designed primer pairs，23 proved to be polymorphic microsatellite markers in the B. lutosa wild populations（WP）.The results showed that the allele number of these microsatellites ranged from 2 to 7.The expected heterozygosity（He）and observed heterozygosity（Ho）ranged from 0.190 to 0.937，and 0.065 to 0.936，respectively.The polymorphism information content（PIC）ranged from 0.061 to 0.777.The He values of the WP and breeding population（BP）were 0.491 and 0.544，respectively.The Ho values of the WP and BP populations were 0.477 and 0.564，respectively.The PIC values for WP and BP were 0.541 and 0.407，respectively.There were 13 population loci that were heterozygote excesses in WP，and 13 population loci in BP.The genetic differentiation index（Fst）ranged from 0.001 to 0.655，with an average value of 0.053（0.05 |
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