文章摘要
趙文飛,李喆,黃小芳,張楊,丁君,常亞青.雜色角孔海膽形態學觀察、親緣關係及性腺營養成分分析.漁業科學進展,2019,40(6):163-172
雜色角孔海膽形態學觀察、親緣關係及性腺營養成分分析
Morphological Observation,Relationship and Nutritional Composition Analysis of Gonadal of Salmacis sphaeroides variegate
投稿時間:2019-02-20  修訂日期:2019-04-25
DOI:
中文關鍵字 雜色角孔海膽  形態學  性腺營養成分  線粒體  16S rDNA
英文關鍵字 Salmacis sphaeroides variegate  Morphology  Gonadal nutrition  Mitochondria  16S rDNA
基金項目
作者組織
趙文飛 大連海洋大學農業農村部北方海水增養殖重點實驗室大連116023 
李喆 大連海洋大學農業農村部北方海水增養殖重點實驗室大連116023 
黃小芳 大連海洋大學農業農村部北方海水增養殖重點實驗室大連116023 
張楊 大連海洋大學農業農村部北方海水增養殖重點實驗室大連116023 
丁君 大連海洋大學農業農村部北方海水增養殖重點實驗室大連116023 
常亞青 大連海洋大學農業農村部北方海水增養殖重點實驗室大連116023 
摘要點擊次數 4496
全文下載次數 5148
中文摘要
      本研究以雜色角孔海膽(Salmacis sphaeroides variegate)為研究資料,觀察其形態,基於線粒體16S rDNA基因部分序列分析雜色角孔海膽與其他棘皮動物的親緣關係,同時分析雜色角孔海膽性腺中的營養成分,為雜色角孔海膽種質改良和資源評估積累數據。結果顯示,雜色角孔海膽的主要形態特徵具有口器較小,每個步帶板的外緣有3~4個小的圓形凹孔,管足壁內的骨片為C形。通過Mega 5.0對其他棘皮動物和得到的雜色角孔海膽的線粒體16S rDNA基因部分序列進行比較,基於距離法(NJ)和最大似然法(MP)分別構建系統樹。結果顯示,雜色角孔海膽與疏棘角孔海膽(Salmacis bicolor rarispina)的親緣關係最近,其次是刻肋海膽屬的3種海膽;雜色角孔海膽性腺中水分為86.1%,灰分為3.0%,粗脂肪為2.0%,蛋白質為8.01%;雜色角孔海膽性腺中的胺基酸種類共有16種,總含量為38.71 g/100 g,EAA/TAA為36.99%,EAA/NEAA為71.84%,DAA/TAA為37.17%;脂肪酸共檢測到15種,含量最高的是棕櫚酸C16:0、硬脂酸C18:0、EPA C20:5和花生四烯酸C20:4,含量最低的為二高-γ-亞麻酸C20:3,較其他海膽,其DHA含量較高。研究結果將為雜色角孔海膽的生物學研究提供基本資料,可用於經濟海膽開發應用,同時也可為遠緣雜交育種及經濟海膽的種質鑒定提供理論依據。
英文摘要
      In this study,Salmacis sphaeroides variegata was used as the research material to observe its morphology.Based on mitochondrial 16S rDNA gene partial sequencing,the genetic relationship between S. sphaeroides variegata and other echinoderms was analyzed.The nutritional composition of S. sphaeroides variegata gonad was analyzed to accumulate a mass of data for germplasm improvement and resource evaluation of S. sphaeroides variegata.The results showed that the main morphological characteristics of the S. sphaeroides variegata were as follows: the apical system was small,the outer edge of each step band plate had 3~4 small circular pits,and the bone fragment in the wall of the pedicle foot was C-shaped.The mitochondrial 16S rDNA gene sequences of other echinoderms and that obtained from S. sphaeroides variegata were compared by Mega 5.0,and the phylogenetic trees were constructed based on distance method(NJ)and maximum likelihood method(MP).The results showed that the S. sphaeroides variegata was closely related to the Salmacis bicolor,followed by the three species of sea urchins,the Temnopleuridae.An amount of water 86.1%,ash 3.0%,crude fat 2.0% and protein 8.01% was found in the sex glands of S. sphaeroides variegata.There were 16 kinds of amino acids in the sex glands of S. sphaeroides variegata,the total amino acid content was 38.71 g/100 g,EAA/TAA was 36.99%,EAA/NEAA was 71.84%,and DAA/TAA was 37.17%.A total of 15 fatty acids were detected,with the highest content being palmitic acid C16:0,stearic acid C18:0,EPA C20:5 and arachidonic acid C20:4,and the lowest content was that of C20:3.S. sphaeroides variegata showed a higher content of DHA than in other sea urchins.The results will provide basic data for the biological study of S. sphaeroides variegata and can be used for the development and application of sea urchin of economic value,as well as provide a theoretical basis for distant hybridization breeding and the germplasm identification of economically viable sea urchins.
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