| 胡鵬莉，吳瑞，魯康樂，王玲，宋凱，張春曉.複合蛋白替代魚粉對花鱸生長、消化能力和腸道健康的影響.漁業科學進展，2019,40（6）：56-65 |
| 複合蛋白替代魚粉對花鱸生長、消化能力和腸道健康的影響 |
| Effects of Replacing Fish Meal with Composite Protein on Growth，Diet Digestibility，and Gut Health in Japanese Seabass（Lateolabrax maculatus） |
| 投稿時間：2018-12-14 修訂日期：2019-01-30 |
| DOI： |
| 中文關鍵字： 複合蛋白 花鱸 生長 消化 腸道組織結構 炎性因數 |
| 英文關鍵字： Composite protein Japanese seabass（Lateolabrax maculatus） Growth performance Digestibility Gut morphology structure Inflammatory genes |
| 基金項目： |
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| 摘要點擊次數： 4171 |
| 全文下載次數： 5010 |
| 中文摘要： |
| 以初均重為（5.80±0.07） g的花鱸（Lateolabrax maculatus）幼魚為實驗對象，研究複合蛋白替代魚粉對花鱸生長、消化和腸道健康的影響。用複合蛋白分別替代對照組飼料中0、20%、40%、60%和80%的魚粉，配製成5種等氮等脂的實驗飼料，分別用這5種飼料投喂花鱸70 d；養殖實驗期間水溫為（29.5±1.5）℃，鹽度為29±1，溶解氧≥6.5 mg/L。養殖實驗結束後採集樣品進行分析，結果顯示，20%替代組花鱸生長效能與對照組無顯著差异（P>0.05），但隨魚粉替代比例的升高，花鱸增重率（WGR）、存活率（SR）、飼料效率（FE）以及蛋白質效率（PER）顯著下降（P<0.05），花鱸腸道蛋白酶活性以及飼料幹物質、粗蛋白與粗脂肪錶觀消化率皆顯著降低（P<0.05），且花鱸後腸黏膜組織結構出現損傷，腸道促炎因數TNF-α、IL-1β基因表達量升高，抗炎因數基因IL-4、IL-10表達量相應降低。研究表明，複合蛋白替代花鱸飼料中魚粉比例不宜超過20%。 |
| 英文摘要： |
| This study was conducted to evaluate the effects of replacing fish meal with composite protein on growth performance，diet digestibility，and gut health in Japanese seabass（Lateolabrax maculatus）.Five isonitrogenous and isolipidic diets were formulated by replacing 0，20%，40%，60%，or 80% of fish meal in the basic diet with composite protein.Quadruplicate groups of 30 fish [initial body weight（5.80±0.07）g] were fed the test diets to apparent satiation twice daily（08:00 and 16:30），and the experiment continued for 70 days.During the feeding trial，water temperature，salinity，and dissolved oxygen values were maintained at（29.5±1.5）℃，29±1，and≥6.5 mg/L，respectively.Samples were collected for analysis after the feeding trial.The main indexes including the growth performance，diet digestibility，intestinal digestive enzymes activities，posterior intestine morphology structure，and expression of inflammation-related genes of the proximal intestine，mid-intestine，and posterior intestine were analyzed to investigate the appropriate replacement proportion of fish meal by composite protein.The results showed that substituting 20% of fish meal with composite protein did not significantly affect growth performance compared to that of the control group（P>0.05），while further increment of fish meal replacement levels resulted in significant reduction of weight gain，survival rate，feed efficiency，and protein efficiency ratio（P<0.05）. Moreover，as the replacement level of fish meal with composite protein exceeded 20%，intestinal protease activity and apparent digestibility coefficients of feed dry matter，crude protein，and crude lipid also significantly decreased with increasing level of fish meal replacement by composite protein（P<0.05）.Meanwhile，increasing replacement of fish meal with composite protein damaged posterior intestine morphology structure and up-regulated expression of gut inflammatory genes such as TNF-αand IL-1β，while an opposite trend was observed for the expression of anti-inflammatory genes，IL-4 and IL-10.In conclusion，it was recommended that the replacement proportion of fish meal with composite protein should not exceed 20%. |
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