| 張永剛，李彬，王印庚，王金燕，廖梅傑，張正，寧魯光，範瑞用.刺參養殖池塘中一株植物乳杆菌的分離及其生物學特性.漁業科學進展，2019,40（6）：154-162 |
| 刺參養殖池塘中一株植物乳杆菌的分離及其生物學特性 |
| Screening and Characteristic Analysis of Lactobacillus plantarum from Ponds for Sea Cucumber Farming |
| 投稿時間：2018-09-12 修訂日期：2018-10-08 |
| DOI： |
| 中文關鍵字： 刺參 腐皮綜合征 燦爛弧菌 假交替單孢菌 植物乳杆菌 抑菌活性 |
| 英文關鍵字： Apostichopus japonicus Skin ulcer syndrome Vibrio splendidus Pseudoalteromonas nigrifaciens Lactobacillus plantarum Antagonistic activity |
| 基金項目： |
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| 摘要點擊次數： 3981 |
| 全文下載次數： 4966 |
| 中文摘要： |
| 2017年5月，從山東東營刺參（Apostichopus japonicus）養殖池塘底泥中分離獲得49株乳酸菌。以刺參“腐皮綜合征”的2株致病菌[燦爛弧菌（Vibrio splendidus）和假交替單胞菌（Pseudoalteromonas nigrifaciens）]為訓示菌進行拮抗作用實驗，獲得1株具有顯著抑菌活性的乳酸菌CLY-5，對該菌株進行了生理生化實驗、16S rDNA序列分析、生長特性及其對刺參的安全性研究。結果顯示，菌株CLY-5對燦爛弧菌和假交替單胞菌具有較好的抑制作用，且菌株的胞內產物與胞外產物均具有抑菌效果，抑菌圈分別為20、23 mm和27、38 mm，胞外產物的拮抗作用優於胞內產物。利用該菌對刺參進行高濃度浸浴測試其安全性，濃度為1×109、1×108和1×107 CFU/ml，實驗期間刺參狀態良好，無死亡現象。16S rDNA序列分析表明，CLY-5與植物乳杆菌（Lactobacillus plantarum NR117813.1）的相似性為100%，初步鑒定該菌株為植物乳杆菌。菌株CLY-5在30℃~44℃、pH 6~8範圍內生長較快，20 h進入對數生長期，28~32 h達到生長高峰。篩選的植物乳杆菌具有良好的抑菌能力，且其生長特性適應刺參池塘的養殖環境，為刺參疾病的生態防控及乳酸菌資源的開發提供應用數據參攷。 |
| 英文摘要： |
| Forty-nine lactic acid bacteria strains were isolated from sediment samples from cultured ponds of sea cucumbers（Apostichopus japonicus）in Dongying，Shandong Province，in May 2017.First，antagonistic bacterial experiments were conducted using Vibrio splendidus and Pseudoalteromonas nigrifaciens，which are the main pathogenic bacteria of“skin ulcer syndrome”for sea cucumbers，and a lactic acid bacteria CLY-5，which has demonstrated significant bacteriostatic activity.Next，physiological and biochemical experiments and a similarity analysis of 16S rDNA sequences as well as the growth character of CLY-5 were carried out.A safety experiment for sea cucumbers was also performed.The results revealed that CLY-5 strain successfully inhibited V. splendidus and P. nigrifaciens.Meanwhile，the strain’s intracellular and extracellular products effectively inhibited V. splendidus and P. nigrifaciens，as demonstrated by inhibition zone diameters of 20 mm，23 mm，and 27 mm，38 mm，respectively，with extracellular products showing superior performance compared with intracellular products.Dipping bath concentrations were set to 1×109，1×108，and 1×107 CFU/ml to test the safety of CLY-5 for sea cucumbers.During the experiment，all sea cucumbers were in good conditions.Based on the 16S rDNA sequence analysis，strain CLY-5 was determined to be identical to Lactobacillus plantarum NR117813.1.In addition，strain CLY-5 exhibited superior growth at 30℃~44℃and salinities of 6 to 8.Moreover，CLY-5 came into a logarithmic growth phase after 20 h of cultivation and reached peak growth after 28~32 h. In summary，strain CLY-5 could be used to reduce the occurrence of sea cucumber disease and is also suitable for pond culture environment.These results provide a basis for ecological prevention strategies in disease control，and should prove useful in the development and utilization of lactic acid bacteria. |
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