| 李炎璐，陳超，陳建國，翟介明.雲紋石斑魚、鞍帶石斑魚及其雜交F1的DNA甲基化分析.漁業科學進展，2019,40（6）：98-104 |
| 雲紋石斑魚、鞍帶石斑魚及其雜交F1的DNA甲基化分析 |
| DNA Methylation Analysis of Epinephelus moara，Epinephelus lanceolatus and Their F1 Hybrid |
| 投稿時間：2018-09-10 修訂日期：2018-10-17 |
| DOI： |
| 中文關鍵字： 雲紋石斑魚 鞍帶石斑魚 雜交F1 DNA甲基化 |
| 英文關鍵字： Epinephelus moara Epinephelus lanceolatus Hybrid F1 DNA methylation |
| 基金項目： |
|
| 摘要點擊次數： 3919 |
| 全文下載次數： 4835 |
| 中文摘要： |
| 為探討石斑魚雜種優勢形成過程中基因組DNA甲基化水准的變化，本研究採用甲基化敏感擴增多態性（Methylation-sensitive amplification polymorphism，MSAP）科技檢測雲紋石斑魚（Epinephelus moara）、鞍帶石斑魚（Epinephelus lanceolatus）及雲紋石斑魚（♀）×鞍帶石斑魚（♂）雜交F1 3個群體的基因組DNA甲基化水准，分析雜交F1與親本基因組DNA甲基化水准的差异。結果顯示，雲紋石斑魚和鞍帶石斑魚的基因組DNA屬於甲基化程度較高的類群；雲紋石斑魚、鞍帶石斑魚及其雜交F1的DNA總甲基化率分別為60.62%、59.38%和55.78%，DNA全甲基化率分別為31.37%、30.67%和29.27%，DNA半甲基化率分別為29.25%、28.71%和26.51%；雜交F1的DNA總甲基化率、全甲基化率和半甲基化率均低於雙親，並存在極顯著差异（P<0.01），3個群體的全甲基化率均大於半甲基化率。研究表明，雲紋石斑魚（♀）×鞍帶石斑魚（♂）雜交F1 DNA甲基化水准與雜種優勢呈負相關，雜交F1 DNA甲基化水准的降低可能是形成快速生長等雜種優勢的原因之一。 |
| 英文摘要： |
| DNA methylation is one of the most important epigenetic modifications.It is vital to maintain the stability of the whole genome and regulate the expression of tissue-specific genes.Methylation level and distribution throughout genomic DNA are significantly correlated with gene expression rate.In order to investigate the changes of genomic DNA methylation level associated with heterosis of Epinephelus spp.，we applied methylation-sensitive amplification polymorphism（MSAP）technology to detect the genomic DNA methylation levels of longtooth grouper（E. moara），giant grouper（E. lanceolatus），and their F1 hybrid [E. moara（♀）×E. lanceolatus（♂）]，and we analyzed the difference of DNA methylation level between the F1 hybrid and parents.The results showed that overall genomic DNA methylation was higher in E. moara and E. lanceolatus.The total methylation rates of E. moara，E. lanceolatus，and the F1 hybrid were 60.62%，59.38%，and 55.78%，respectively.The rates of fully methylated sites were 31.37%，30.67%，and 29.27%，respectively，and the hemi-methylation rates were 29.25%，28.71%，and 26.51%，respectively.The total methylation rate，the full methylation rate，and the hemi-methylation rate of DNA of the F1 hybrid were all significantly lower（P<0.01）than that of either parent.The full methylation rates were all higher than the hemi-methylation rates of E. moara，E. lanceolatus，and the F1 hybrid. The study also showed that the DNA methylation level of the F1 hybrid is negatively correlated with heterosis.Moreover，reduced DNA methylation level of the F1 hybrid may be one of the reasons for the heterosis phenomena such as rapid growth.In general，DNA methylation is negatively correlated with gene expression.The DNA methylation level of the hybrid was lower than either parent，allowing otherwise silenced genes to be activated，enhancing gene expression activity，and resulting in the hybrid progeny exhibiting heterosis.The relationship between methylation level of specific genes and heterosis，the genetic pattern of these DNA methylation sites，the effect on gene structure and function，and the regulatory mechanism require further study. |
| 附件 |
|
查看全文 查看/發表評論 下載PDF閱讀器 |
| 關閉 |
