關健,官曙光,鄭永允,慕偉,劉洪軍.2-苯氧乙醇和丁香酚對許氏平鮋幼魚麻醉效果的實驗研究.漁業科學進展,2010,31(6):22-28 |
2-苯氧乙醇和丁香酚對許氏平鮋幼魚麻醉效果的實驗研究 |
Effects of 2-Phenoxyethanol and Clove Oil as Anaesthetics to Sebastes schlegeli Juvenile |
投稿時間:2009-12-01 修訂日期:2010-01-25 |
DOI: |
中文關鍵字: 許氏平鮋 2-苯氧乙醇 丁香酚 麻醉 呼吸頻率 |
英文關鍵字: Sebastes schlegeli 2-Phenoxyethanol Clover oil Anaesthetics |
基金項目:海洋公益性行業科研專項經費項目(No.200805069)、山東省良種工程“優質抗病速生魚類良種選育” |
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摘要點擊次數: 9968 |
全文下載次數: 9675 |
中文摘要: |
研究了2-苯氧乙醇、丁香酚麻醉2個規格許氏平鮋幼魚的效果。幼魚呼吸頻率隨麻醉劑濃度的上升而下降顯著(P<0.001),在高濃度液(2-苯氧乙醇:500、600、800μL/L;丁香酚:60、80μL/L)中可在短時間內降低為0(休克),在中濃度液(2-苯氧乙醇:400μL/L;丁香酚:30、40μL/L)中存在不同程度下降,而低濃度液(2-苯氧乙醇:200、300μL/L;丁香酚:10、20μL/L)對呼吸頻率總體影響較小。同等濃度麻醉劑對小規格幼魚呼吸頻率的影響(P<0.01)高於大規格幼魚。2-苯氧乙醇中大規格幼魚的入麻、休克和復蘇時間均長於小規格幼魚(P<0.05)。大規格幼魚對麻醉劑的耐受能力强於小規格幼魚;復蘇能力方面,丁香酚麻醉的大規格幼魚復蘇遲於小規格幼魚,但2-苯氧乙醇麻醉的大、小規格幼魚復蘇時間無差异。綜合考慮入麻、休克、復蘇時間和死亡率,認為使用2-苯氧乙醇、丁香酚麻醉許氏平鮋幼魚的理想濃度分別為300μL/L、20μL/L。 |
英文摘要: |
Schlegel's rockfish(Sebastes schlegeli),distributed in the western North Pacific,is an important offshore cage-culture fish species in the Yellow Sea and Bo-hai Sea of China.Schlegel's rockfish juvenile(TL=55.2mm,99.0mm,short in SJ and LJ,respectively)were exposed to seawater containing different concentrations of 2-Phenoxyethanol from 200mg/L to 800mg/L,clove oil from 10mg/L to 80mg/L. The respiratory rates and anaesthetic effect of 2-Phenoxyethanol and clove oil were studied.Normally,the respiratory rate of SJ(22.8/10s)was higher than LJ(19.7/10s).The respiratory rate descended remarkably(P<0.001)as the anaesthetic concentration ascended: it would be descended to 0 in short time(shock stage)in high concentration(2-Phenoxyethanol: 500,600,800μL/L;clove oil: 60,80μL/L),descended in different levles in middle concentration(2-Phenoxyethanol: 400μL/L;clove oil: 30、40μL/L),and it has affected mildly in low concentration(2-Phenoxyethanol: 200,300μL/L;clove oil: 10,20μL/L).The effects on respiratory rate of SJ were more remarkable than of LJ in the same concentrations(P<0.01).The anaesthetic time,shock time and recovery time of LJ were longer than SJ in 2-Phenoxyethanol.The anti-anaesthetic ability of LJ was stronger than SJ. In recovery ability,the LJ recoveried later than SJ in clove oil seawater,but there was not the similar phenomenon been seen in 2-Phenoxyethanol.By thought of anaesthetic time,shock time,recovery time and mortality,the optimum concentrations of 2-Phenoxyethanol and clove oil were 300μL/L,20μL/L for use. |
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