Standardized procedures and acceptance specifications for sterile rooms
Standardized procedures and acceptance specifications for sterile rooms The sterile room is usually a small room in the microbiological laboratory. Color steel plate and tempered glass shall be used for construction. The area should not be too large, about 5-20 square meters, about 2.5 meters high. A buffer room shall be set outside the sterile room, and the door of the buffer room and the door of the sterile room shall not face the same direction, so as to avoid air flow bringing in foreign bacteria. The aseptic room and buffer room must be sealed. The indoor air exchange equipment must be equipped with air filtration device. The floor and wall of the sterile room must be flat, not easy to hide dirt and dirt, and easy to clean. The table top of the workbench should be horizontal. Both the aseptic room and the buffer room are equipped with ultraviolet lamps, and the ultraviolet lamps in the aseptic room are 1m away from the work table. The staff entering the sterile room should wear sterilized clothes and hats. At present, aseptic rooms are mostly found in microbial factories, and general laboratories use ultra clean tables. The main function of the ultra cleaning table is to use the air laminar flow device to remove all kinds of micro dust, including microorganisms, from the upper part of the worktable. The air enters the working table after passing through the high-efficiency filter through the electric device to keep the table under the control of flowing sterile air. In addition, there is a high-speed flow air curtain on the side close to the outside to prevent the external bacteria carrying air from entering. At present, aseptic manipulation technology not only plays an important role in microbiology research and application, but also is widely used in many biotechnology. For example, transgenic technology, monoclonal antibody technology, etc.