Primer Premier v6.0+v6.25 trial version

Primer Premier It is a software developed by Premier Company in Canada, which is specialized in the design and evaluation of PCR or sequencing primers and hybridization probes. Its main interface is divided into sequence editing window (Genebank), primer design window (Primer Design), restriction sites window (Restriction Sites) and motif analysis window (Motif).

Primer Premier software has four main functions, namely primer design, restriction endonuclease site analysis, DNA motif search and homology analysis. The first three are its main functions. In addition, the software has some special functions, the most important of which is the design of degenerate primers. In addition, there are sequence "reading", the exchange of DNA and protein sequences, voice prompt keyboard input, and so on.

Primer Premier software can also convert DNA and amino acid sequences with corresponding genetic code rules according to the source of template DNA. The software provides users with different genetic code rules for eight biological substructures, including ciliate macronucleus, invertebrate mitochondrion, mycoplasma, plant mitochondrion, protozoan mitochondrion, standard Vertebrate Mito chondrion and Yeast Mitochondrion.

Primer Premier software has excellent automatic primer search function, and can analyze some indicators, and is easy to use. It has been considered as a very good software by the primer design industry.

A Comprehensive PCR Primer Design Software

Primer Premier’s search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.

Primer Design for Standard PCR Assays

Primer Premier is the most comprehensive software to design and analyze PCR primers.

Primer Premier’s search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor thermodynamic algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in ranked order. Equipped with a handy calculator, you can easily manipulate sequences and analyze the results of your primer design.

Primer Design for SNP Genotyping Assays

With Primer Premier, you can load sequences from dbSNP and have the primers designed flanking the SNP selected. Hundreds of unpublished SNPs can also be loaded by specifying them as variation features in standard GenBank/dbSNP files. After specifying the SNPs, primers can be designed to amplify them for detection using a probe-based chemistry.

Official website: http://www.premierbiosoft.com/primerdesign/index.html

Download address

Biological PCR sequencing primer and hybridization probe Primer Premier v6.0+v6.25 trial version
https://www.123pan.com/s/PjcA-KbORA.html
http://ctfile.luochenzhimu.com/d/13552432-58890970-9decd3
https://pan.baidu.com/s/18pLFHmB_SRDVC-yKMvV2eg?pwd=59lm

Installation Guide:
Run and install Primer Premier 6.0 Installer.exe
Replace the RMToolkit.jar in the directory C: Program Files (x86) Primer Premier 6.0 http Dwwww. premierbiosoft. com P80 DMIUpdater DMPP DM600 with Crack RMToolkit.jar
If you change the installation directory, you can follow the changed path to find RMToolkit.jar to replace

Primer Premier 6.25 trial download:
Win32： https://www.premierbiosoft.com/DATAFILES/PP625InstallerWin32.exe
Win64： https://www.premierbiosoft.com/DATAFILES/PP625InstallerWin64.exe
Mac： https://www.premierbiosoft.com/DATAFILES/PP625Installer.dmg

Primer Premier 6.24 Trial download:
Win32： https://www.premierbiosoft.com/DATAFILES/PP624InstallerWin32.exe
Win64： https://www.premierbiosoft.com/DATAFILES/PP624InstallerWin64.exe
Mac： https://www.premierbiosoft.com/DATAFILES/PP624Installer.dmg