Full automatic anaerobic workstation ZDYY-II Anaerobic incubator is also called anaerobic workstation or anaerobic glove box. Anaerobic incubator is a special device for bacteria cultivation and operation in anaerobic environment. It can provide strict anaerobic state and constant temperature cultivation conditions and has a systematic and scientific working area.
Now let's learn about the detailed methods of placing bacteria and cultivating bacteria in the anaerobic incubator
1) Check and close the door in the sampling room.
2) Open the door outside the sampling room, put the bacteria into the sampling room, and then close the door.
3) During the three times of nitrogen filling replacement in the sampling room, first pump the vacuum above 500mm Hg (66Kpa) and stop, then open the oxygen valve in the sampling room to intake air, make the pointer return to zero, and then close the valve. The three nitrogen replacement processes in the sampling room are completed.
4) If the vacuum degree is selected to be low, the replacement times need to be increased.
5) After the door outside the sampling room is opened and closed tightly, the vacuum is pumped down to 100 mm Hg (13 Kpa) for inspection.
6) Conditions for long-term continuous use of anaerobic incubator
(1) Put Meilan indicator strip in the operation room every day for observation. If it is not normal, you must breathe again.
(2) A small amount of mixed gas shall be continuously input for a long time. The added nitrogen can be combined with the trace nitrogen through catalytic absorption to ensure the indoor anaerobic state. The flow rate of the added mixed gas is about 10 ml per minute.
(3) Replace the deoxidizer and desiccant once after continuous cultivation for one day. (The replaced deoxidizer and desiccant can be put in a 200 ℃ drying oven and can be recovered after drying for 2-3 hours)
7) Application of inoculation stick sterilizer:
(1) Use the nickel soldering electric wire at any time to short-circuit the electrode and turn red hot for sterilization (inoculation rod).
(2) The wax melting position can be directly put on the tube sealing mouth to rotate the wax melting.
8) The temperature in the incubator of the operating room can be selected and controlled at will.
Full automatic anaerobic workstation ZDYY-II Anaerobic incubator is one of the commonly used equipment in the laboratory. In order to use the anaerobic incubator longer, we should pay attention to maintenance in addition to use.
Operation method of anaerobic incubator:
1. Check and close the door in the sampling room;
2. Open the door outside the sampling room, put the bacteria into the sampling room, and then close the door;
3. Three nitrogen replacement processes in the sampling room: open the vacuum pump, pump the vacuum above 500ml Hg (66kPa) and stop, then manually open the nitrogen valve 2 for air intake, make the pointer return to zero, and close the valve 2. Repeat the operation for the second time. In the third operation, stop the vacuum above 500ml Hg (66kPa), then open valve 4 to feed the mixture, return the pointer to zero, and close valve 4. The three nitrogen replacement processes in the sampling room are completed;
4. If the selected vacuum degree is low, the replacement times need to be increased;
5. Open and close the door outside the sampling room, and then pump 100ml Hg (13kPa) at low vacuum for inspection;
6. Adjustment of output pressure of mixing gas cylinder and nitrogen cylinder: adjust the pressure reducing valve to make the output pressure about 0.1Mpa.
Conditions for long-term use of anaerobic incubator:
1. Open the Meilan indicator paper in the operation room every day for observation. If it is abnormal, it must be re ventilated;
2. It is necessary to continuously input a small amount of mixed gas for a long time, so that the added nitrogen can be combined with a small amount of oxygen through catalytic absorption to ensure the indoor anaerobic state, and the flow rate of the added mixed gas is about 10ml per minute;
3. Run the culture continuously for one day, and replace the deoxidizer and desiccant once.
1. As far as possible, it should be installed in a place with quiet air and little temperature change.
2. Before starting the machine, you should be fully familiar with and understand the instructions of each supporting instrument and anaerobic incubator, and master the correct use methods.
3. The culture must be put into the operating room after reaching the anaerobic environment.
4. In the process of using the anaerobic incubator, do not add too many articles in the box to avoid affecting the temperature uniformity in the box, and do not block the circulating fan and air duct.
5. In case of failure (gas stop and other reasons), the operating room can still remain anaerobic for 12 hours.
6. Always pay attention to whether there is air leakage in the air path of the anaerobic incubator. When replacing the gas cylinder, pay attention to tightening the gas pipe to avoid flowing into oxygen containing gas. The vacuum pump shall be used as required, and the oil filling shall be checked regularly.
7. Flammable, explosive and corrosive materials shall not be placed in the box. When using the anaerobic incubator, it is necessary to leave some heat dissipation space outside the incubator, otherwise the cooling effect will be affected.
8. Please turn off the main power button and the power switch on the back of the device when it is out of use.
9. When the incubator is out of use, the interior of the incubator should be dried as follows: the incubator temperature should be set above 40 ℃, the incubator should run stably for two hours, the incubator door should be opened for 10 minutes every half an hour, the incubator door should be closed, and the incubator door should be operated repeatedly for 3-5 times in sequence.
Several points that cannot be ignored when using anaerobic incubator
Anaerobic incubator is also called anaerobic workstation or anaerobic glove box. The compact cylindrical design is adopted to realize the easy transfer of samples by one person and one hand. It is generally composed of thermostatic incubation room, anaerobic operation room, sampling room, gas circuit and circuit control system, box rack, bottle rack, wax melting sterilizer, etc. Mechanical forced convection and positive pressure in the inner chamber realize stable and uniform state in four aspects of constant temperature, humidity control, deoxygenation and biological detoxification, and recover quickly. The operation and cultivation are carried out in the same room. It is a special device for bacteria cultivation and operation in anaerobic environment, which can cultivate anaerobic organisms that are difficult to grow and avoid the danger of death when anaerobic organisms are exposed to oxygen when operating in the atmosphere. Therefore, the device is an ideal tool for anaerobic biological detection research. It is a special device for bacteria culture and operation in anaerobic environment. It can provide strict anaerobic state and constant temperature cultivation conditions and has a systematic and scientific working area. Relevant technicians said that the following details should not be ignored when using anaerobic incubator:
1. The culture in the anaerobic incubator must be put into the operation room after reaching the absolute dui anaerobic environment.
2. When replacing the gas cylinder, pay attention to tighten the gas pipe to avoid flowing into oxygen containing gas
3. The vacuum pump shall be used as required, and the oil filling shall be checked regularly.
4. The whole machine shall be placed at a place with small temperature difference and convenient operation. It shall be kept away from direct sunlight and heating equipment and placed stably.
5. Stop using, turn off the main power key and the power switch at the rear of the equipment.
6. Before starting the machine, you should be familiar with and understand the instructions of the supporting instruments and meters, and master the correct use methods.
7. The instrument shall be installed in the place with quiet air and small temperature change as far as possible.
8. In case of failure (gas stop and other reasons), the operating room can still remain anaerobic for 12 hours. (If it exceeds 12 hours, take out the culture for another treatment as required).
9. The anaerobic incubator should always pay attention to whether there is air leakage in the air path.
