Anaerobic glove box YQX-III micro aerobic environment
Anaerobic culture system has more advantages than anaerobic incubator, mainly in the following aspects:
1) The gas consumption is very small. Generally, a bottle of gas in the anaerobic operation box will be used up in less than one or two times, hundreds of yuan!
2) The operation is very fast, it only takes about 1 minute to reach the micro aerobic state, and it only takes about 2 minutes for anaerobic operation, which is beyond the anaerobic operation box
3) The repeatability is particularly good. Because of the reliability of the device, the results are the same every time, and the possibility of being affected by external factors is almost zero. With the anaerobic operation box, we can imagine how difficult it is to reach the same state every time in such a large space.
4) Compared with the size, the anaerobic culture system is very light and easy to move. Generally, women working in the laboratory are easy to move. Anaerobic incubator occupies a large volume and is not generally inconvenient to move.
Anaerobic glove box YQX-III micro aerobic environment
Anaerobic incubator is one of the commonly used equipment in the laboratory. In order to use the anaerobic incubator longer, we should pay attention to maintenance in addition to use.
Operation method of anaerobic incubator:
1. Check and close the door in the sampling room;
2. Open the door outside the sampling room, put the bacteria into the sampling room, and then close the door;
3. Three nitrogen replacement processes in the sampling room: open the vacuum pump, pump the vacuum above 500ml Hg (66kPa) and stop, then manually open the nitrogen valve 2 for air intake, make the pointer return to zero, and close the valve 2. Repeat the operation for the second time. In the third operation, stop the vacuum above 500ml Hg (66kPa), then open valve 4 to feed the mixture, return the pointer to zero, and close valve 4. The three nitrogen replacement processes in the sampling room are completed;
4. If the selected vacuum degree is low, the replacement times need to be increased;
5. Open and close the door outside the sampling room, and then pump 100ml Hg (13kPa) at low vacuum for inspection;
6. Adjustment of output pressure of mixing gas cylinder and nitrogen cylinder: adjust the pressure reducing valve to make the output pressure about 0.1Mpa.
Conditions for long-term use of anaerobic incubator:
1. Open the Meilan indicator paper in the operation room every day for observation. If it is abnormal, it must be re ventilated;
2. It is necessary to continuously input a small amount of mixed gas for a long time, so that the added nitrogen can be combined with a small amount of oxygen through catalytic absorption to ensure the indoor anaerobic state, and the flow rate of the added mixed gas is about 10ml per minute;
3. Run the culture continuously for one day, and replace the deoxidizer and desiccant once.
The use process of anaerobic incubator is actually two steps: the formation of anaerobic environment in the operating room and the placement and cultivation of bacteria, but each step requires many small steps to complete. Let's take a look at it together.
1、 Formation of anaerobic environment in operation room:
Place the necessary accessories and appliances according to the use requirements, and put two five toxic plastic bags into the operation room. After the equipment is turned on, press the main power button on the control board to power on the equipment. First, insert a rubber tube into the air inlet of the operating room, and the other into a plastic bag, connect the nitrogen air inlet circuit, open the nitrogen control valve of the operating room of the anaerobic incubator, make two plastic bags full of nitrogen, close the solenoid valve, then tighten the bag mouth, put latex gloves on the flange ring of the observation plate and tighten them, and gradually put the nitrogen in the plastic bag into the operating room until it is completely prevented. In the operating room of the anaerobic incubator, turn on the palladium particle deoxidizer, connect the power supply of the deoxidizer catalyst for catalytic deoxidization, and drop a little clean water on the reaction area of the anaerobic indicator strip. After 1-2 hours, observe its change. When the reaction part turns dark blue to light blue, the operating room reaches the anaerobic environment.
2、 Insertion and culture of strain:
Check whether the door in the sampling room of the anaerobic incubator is closed tightly, open the door outside the sampling room, close the outside door after putting the bacteria into the sampling room, fill the sampling room with nitrogen for three times, then open the oxygen valve in the sampling room to let the pointer return to zero, and close the valve. Anaerobic incubator needs the condition of long-term continuous use. Put an indicator strip in the operation room every day for observation. If it is not normal, it must be re ventilated. It is necessary to continuously input a small amount of mixed gas for a long time, so that the supplemented nitrogen can combine with a small amount of nitrogen through catalytic absorption, ensuring the anaerobic state in the room. The flow rate of the supplemented mixed gas is selected to be about 10 ml per minute, After continuous cultivation for one day, replace the deoxidizer and desiccant once.
Several points that cannot be ignored when using anaerobic incubator
Anaerobic incubator is also called anaerobic workstation or anaerobic glove box. The compact cylindrical design is adopted to realize the easy transfer of samples by one person and one hand. It is generally composed of thermostatic incubation room, anaerobic operation room, sampling room, gas circuit and circuit control system, box rack, bottle rack, wax melting sterilizer, etc. Mechanical forced convection and positive pressure in the inner chamber realize stable and uniform state in four aspects of constant temperature, humidity control, deoxygenation and biological detoxification, and recover quickly. The operation and cultivation are carried out in the same room. It is a special device for bacteria cultivation and operation in anaerobic environment, which can cultivate anaerobic organisms that are difficult to grow and avoid the danger of death when anaerobic organisms are exposed to oxygen when operating in the atmosphere. Therefore, the device is an ideal tool for anaerobic biological detection research. It is a special device for bacteria culture and operation in anaerobic environment. It can provide strict anaerobic state and constant temperature cultivation conditions and has a systematic and scientific working area. Relevant technicians said that the following details should not be ignored when using anaerobic incubator:
1. The culture in the anaerobic incubator must be put into the operation room after reaching the absolute dui anaerobic environment.
2. When replacing the gas cylinder, pay attention to tighten the gas pipe to avoid flowing into oxygen containing gas
3. The vacuum pump shall be used as required, and the oil filling shall be checked regularly.
4. The whole machine shall be placed at a place with small temperature difference and convenient operation. It shall be kept away from direct sunlight and heating equipment and placed stably.
5. Stop using, turn off the main power key and the power switch at the rear of the equipment.
6. Before starting the machine, you should be familiar with and understand the instructions of the supporting instruments and meters, and master the correct use methods.
7. The instrument shall be installed in the place with quiet air and small temperature change as far as possible.
8. In case of failure (gas stop and other reasons), the operating room can still remain anaerobic for 12 hours. (If it exceeds 12 hours, take out the culture for another treatment as required).
9. The anaerobic incubator should always pay attention to whether there is air leakage in the air path.
