Analysis of mRNA 5 'capping rate

MRNA 5 'capping refers to the process of attaching a 7-methylguanosine (m7G) cap to the 5' end of a newly synthesized mRNA molecule. It is one of the key steps in eukaryotic gene expression and is crucial for the stability, output, translation and degradation of mRNA. The analysis of mRNA capping rate is a method to evaluate whether the 5 'end of mRNA molecule is modified by m7G caps. The analysis of mRNA capping rate not only plays an important role in basic biological research, but also has potential applications in disease diagnosis and treatment. For example, many diseases (including some types of cancer) are related to abnormalities in the process of mRNA capping, which is also a key component of modern mRNA vaccines, such as COVID-19 vaccine.

 



MRNA molecule with m7G cap at 5 'end

 

M7G modified 5 'capping will increase the mass of RNA molecules, and the mass spectrum can detect this change. In the mass spectrum, RNA molecules with 5' capping will show a larger m/z value peak than unmodified RNA molecules. By measuring the relative strength of these two peaks, the 5 'capping rate can be calculated. BTP has passed the CNAS/ISO9001 double quality system certification. The company has established an mRNA 5 'capping rate analysis platform using Thermo's Orbitrap Fusion Lumos mass spectrometer combined with Nano LC nanoliter chromatography technology to provide you with one-stop mRNA 5' capping rate analysis service. Just tell us your experiment purpose and send samples. Biotech is responsible for all project follow-up. For more details, please consult for free!

 

Project report in Chinese/English

In the technical report, Patek will provide you with a detailed technical report in both Chinese and English, including:

1. Experiment steps (Chinese and English)
2. Relevant mass spectrum parameters (Chinese and English)
3. Details of mRNA capping rate analysis
4. Mass spectrum picture
5. Original data

 

Analysis of mRNA capping rate One stop service

You only need to place an order - send samples
One stop service of BTP: sample processing - computer analysis - data analysis - project report

 

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