Anther culture in vitro is a tissue culture technology, and its process is:
1. The anthers with pollen developed to a certain stage are inoculated on the artificial medium for in vitro culture through aseptic operation technology;
2. Pollen can divide many times under the specific conditions provided by the culture medium, forming callus similar to embryonic structure;
3. The callus was induced to differentiate into buds and roots, and finally grew into plants.
Haploid breeding is a breeding method, and its process is:
On the basis of anther culture in vitro, haploid seedlings were treated with colchicine to double the number of chromosomes and restore to double. Because their diploid chromosomes are doubled from the uniploid chromosomes themselves, they are all pure lines, and the self bred offspring will not have character separation, so they have high application value in breeding.